An Experimental Study on Development of Cultured Epithelial Grafting Sheets with Amnion and Oral Epithelial Cells

点击翻译按钮获取中文摘要

The aim of this study was to assess the possibility of human oral mucosal epithelial cells on hyper-dry-amnion can be used as new source for culturing stratified epithelium sheets. The oral keratinocyte and hyper-dry-amnion composites had a well-stratified epithelial layer similar to native oral keratinized mucosa. Immunohistochemistry, the oral keratinocyte and hyper-dry-amnion composites appeared to be in a more proliferative state than native keratinized mucosa. These results suggested that the oral keratinocyte and hyper-dry-amnion composites may be a promising cell source for the development of stratified epithelium allograft sheets using a human cell strain.