A biocomposite material coordinated with a biopolymer hydrogel and mesenchymal stem cells for bone repair

与生物聚合物水凝胶和间充质干细胞配合用于骨修复的生物复合材料

• 批准号: 20791477
• 负责人: SHIBATA Yo
• 金额: $ 2.83万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791477/
• 关键词: リン酸カルシウム; コラーゲン; 骨充填材料; 幹細胞; インプラント

The biomechanical stability of osseointegrated implants is of particular importance, especially the stability which is achieved from structural manipulation at the interface between the implant surface and the bone tissues. Nanoscale b-tricalcium phosphate-immobilized titanium was prepared by discharge into a physiological buffered saline solution. Compared with hydroxyapatite, it has been shown to be effective in generating a bone-like chemical structure on the surface by cooperative interaction between osteoblastic cells and the b-tricalcium phosphate. The present study, after cell cultivation, investigates the nanostructures and biomechanical property differences of a mineralized layer formed on two samples of nano-calcium phosphate-immobilized titanium. A scanning probe microscope study revealed that the mineralized tissue formed on the b-tricalcium phosphate samples after 1 week of cell culture showed significantly higher roughness, compared with hydroxyapatite samples. Nanoindentation micromechanical evaluation of the in vitro generated multilayered structures exhibited thicker bone-like mineralized layers on the β-tricalcium phosphate samples. A successful modification of titanium implants through the cooperative interaction between osteoblastic cells and nano β-tricalcium phosphate is anticipated.

骨整合种植体的生物力学稳定性尤为重要，尤其是种植体表面与骨组织界面的结构操纵所获得的稳定性。通过在生理盐水中放电的方法制备了纳米磷酸三钙固定钛。与羟基磷灰石相比，它通过成骨细胞与b-磷酸三钙之间的协同作用，在表面形成了类似骨的化学结构。本研究通过细胞培养，研究了两种纳米钙磷酸盐固定钛表面形成的矿化层的纳米结构和生物力学性能的差异。扫描探针显微镜观察发现，细胞培养1周后，β-磷酸三钙表面形成的矿化组织粗糙度明显高于羟基磷灰石。对体外生成的多层结构的纳米压痕微观机械评估显示，β-磷酸三钙样品上有更厚的骨样矿化层。通过成骨细胞和纳米β-磷酸三钙之间的协同作用，有望成功地对钛植入物进行修饰。

项目成果

Micromechanical evaluation of mineralized multilayer

矿化多层膜的微观力学评价

• 发表时间: 2008
• 期刊: J Biomech 41
• 作者: Yo Shibata;Li Hong He;Yuriko Toda;Yu Kataoka;Naoki Fujisawa;Takashi Miyazaki;Michael V. Swain
• 通讯作者: Michael V. Swain

Micro-CT-based Colorimetry of Enamel Subjected to Bleaching Agents

基于显微 CT 的漂白剂牙釉质比色法

• 发表时间: 2009
• 作者: Tanaka R.;Shibata Y.;Manabe A.;Mipazaki T.
• 通讯作者: Mipazaki T.

Micro-organisms and cell viability on antimicrobial modified titanium.

抗菌改性钛上的微生物和细胞活力。

• 发表时间: 2009
• 期刊: J Dent Res 88
• 作者: S.Omori;Y.Shibata;T.Arimoto;T.Igarashi;K.Baba;T.Miyazaki
• 通讯作者: T.Miyazaki

インターフェースインプラントロジー

界面种植学

• 发表时间: 2008
• 作者: Tanaka R.;Shibata Y.;Manabe A.;Mipazaki T.;柴田陽
• 通讯作者: 柴田陽

Mineralization potential of polarized dental enamel

极化牙釉质的矿化潜力

• 发表时间: 2009
• 期刊: PLoS One
• 影响因子: 3.7
• 作者: Tanaka R.;Shibata Y.;Manabe A.;Miyazaki T
• 通讯作者: Miyazaki T