Analysis of signaling pathway of periostin gene expression by mechanical stress on periodontal ligament cells.

Rho激酶抑制剂Y27632对人牙周膜细胞的碱性磷酸酶活力和矿化能力有抑制作用。此外，添加机械应力的Y27632组比不添加机械应力的组受到抑制。综上所述，这些结果表明，机械应力和Y27632可能更多地上调了Periostin基因的表达，进一步促进了成纤维细胞的分化。

The alkaline phosphatase activity and the ability of mineralization on the human periodontal ligament(HPDL) cells was suppressed by Y27632, that is Rho kinase inhibitor. Additionally, the groups that is added Y27632 with mechanical stress was suppresser than that without mechanical stress. In consideration of the precious research, these results show mechanical stress and Y27632 may more upregulate periostin gene expression and more accelerate the differentiation of fibroblastic cells.