接着分子応用による付着上皮接着機構制御

应用粘附分子控制粘附上皮粘附机制

• 批准号: 20791620
• 负责人: 衣松 高志
• 金额: $ 1.58万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791620/
• 关键词: laminin-5; integrin-α6β4; レーザーマイクロダイセクション; 付着上皮

本年度の研究計画に従い、正常上皮および創傷治癒時における歯-上皮接着部のタンパク発現を正確に捕らえることを目的とした。具体的にはIn vivoにおける接着・移動関連タンパクの検索において、特に正常組織における接着タンパクおよび細胞移動に関わるタンパク(laminin-5、integrinα6β4、integrinα3β1)について検索を行ない、同タンパク質についてはレーザーマイクロダイセクションによる基底細胞の採取によりその発現量が基本的な基底細胞である結合織-上皮問のものと異なり、1aminin-5および細胞の異動に関与するintegrinα3β1の発現が高いことを証明した。この際laminin-5の発現量が多いという事実は、collagen Type4等の基底膜の基本的な構成要素を欠く歯一歯肉上皮、つまり無機質と有機質という特殊な接着部位における接着タンパクの構成量は通常の基底膜とは異なることを示している。なお歯周組織の病態ごとの発現検索においてはマウス臼歯歯間部に対して縫合糸を挿入することにより歯周疾患を誘発し、その破壊過程における細胞間接着タンパク、基底膜状上の接着タンパクおよび細胞間接着タンパク(コネキシン、カドヘリン)に関して検索を進めた。この際、観察時期は1日、3日、1週、2週、1ヶ月2ヶ月とし、実験動物にはICRマウス(5〜6w)を用いた。この実験については本年度は免疫組織学的な検索を中心に行ない、方法としては免疫蛍光染色を用いた。同検索においては炎症波及時の接着の破壊および治癒時におけるlaminin-5の多量の分泌が観察された。これらの結果については来年度予定している培養細胞での検索においてさらに検討する予定である。

In this year's research program, the normal epithelium was used during the treatment period. The epithelial junction was used to make sure that the target was correct. The specific In vivo information will then be moved to the normal tissue, and then to the normal tissue and then to the laminin-5, integrin α 6 β 4, integrin α 3 β 1). In the same way, the basal cell of the basal cell is used to detect the activity of integrin α 3 β 1. In the same way, the basic basal cell is used to combine the tissue and the epithelium of the basal cell, the activity of integrin α 3 β 1 and the activity of integrin α 3 β. The basic components of the basement membrane, such as laminin-5, collagen Type4, and so on, are not suitable for the meat epithelium, the machine-free machine, the special position, the weight, the weight, the temperature, the temperature and the temperature. In the tissue of the disease, it was found that there was an increase in the incidence of disease and disease in the tissue of the disease, and then in the cell of the disease, and then in the membrane of the base, and then in the membrane of the base. The first, 3rd, 1st, 3rd, 1st, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 3rd, 3rd, 1st, 3rd, 1st, 3rd, 1st, 3rd, 1st, 3rd, 1st, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 2nd, 1st, 3rd, 1st, 2nd This year, the immunology center of immunology has been tested, and the methods have been used to immunize light staining. In the same period of time, the inflammation was affected, and then the laminin-5 was detected during the treatment. The results show that there is a forecast for the coming year. Please do not know how to prepare for the coming year.