权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The functional analysis of exposure to a MRI-type high-strength static magnetic field stimulates megakaryocytic/erythroid hematopoiesis in CD34+ cells from human placental and umbilical cord blood

暴露于 MRI 型高强度静磁场刺激人胎盘和脐带血 CD34 细胞巨核细胞/红细胞造血的功能分析

基本信息

批准号：
20890012
负责人：
MONZEN Satoru
金额：
$ 2.11万
依托单位：
Hirosaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (Start-up)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2009
项目状态：
已结题

项目摘要

The biological response after exposure to a high-strength static magnetic field (SMF) has recently been widely discussed from the perspective of possible health benefits as well as potential adverse effects. To clarify this issue, CD34^+ cells from human placental and umbilical cord blood were exposed under conditions of high-strength SMF in vitro. The high-strength SMF exposure system was comprised of a magnetic field generator with a helium-free superconducting magnet with built-in CO_2 incubator. Freshly prepared CD34 cells were exposed to a 5 tesla (T) SMF with the strongest magnetic field gradient (41.7T/m) or a 10 T SMF without magnetic field gradient for 4 or 16 h. In the harvested cells after exposure to 10 T SMF for 16 h, a significant increase of hematopoietic progenitors in the total burst-forming unit erythroid- and megakaryocytic progenitor cells-derived colony formation was observed, thus producing 1.72- and 1.77-fold higher than the control, respectively. Furthermore, early hematopoiesis-related and cell cycle-related genes were found to be significantly up-regulated by exposure to SMF. These results suggest that the 10 T SMF exposure may change gene expressions and result in the specific enhancement of megakaryocytic/erythroid progenitor (MEP) differentiation from pluripotent hematopoietic stem cells and/or the proliferation of bipotent MEP.

暴露于高强度静磁场（SMF）后的生物学反应最近已从可能的健康益处以及潜在的不良反应的角度进行了广泛讨论。为了阐明这一问题，将来自人胎盘和脐带血的CD 34 ^+细胞暴露于体外高强度SMF条件下。高强度SMF照射系统由无氦超导磁体磁场发生器和内置CO_2培养箱组成。将新鲜制备的CD 34细胞暴露于具有最强磁场梯度（41.7T/m）的5 T SMF或无磁场梯度的10 T SMF中4或16 h。在收获的细胞暴露于10 T SMF 16 h后，观察到总爆发形成单位红细胞和巨核细胞祖细胞衍生的集落形成中造血祖细胞的显著增加，从而分别产生比对照高1.72和1.77倍。此外，早期造血相关和细胞周期相关的基因被发现显着上调暴露于SMF。这些结果表明，10 T SMF暴露可能会改变基因表达，并导致多能造血干细胞的巨核细胞/红系祖细胞（MEP）分化和/或双能MEP增殖的特异性增强。