Elucidation of mechanisms for the decrease in kallikrein secretion in isolated renal connecting tubular cells and examination of salt sensitivity on polymorphisms

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(1) We have established a calcium imaging model on primary cultured rat renal connecting tubules. Vasopressin increases intracellular calcium via a V1a receptor. (2) We have tried to obtain monoclonal cells of the kallikrein secreting cell by maximum diluting cultured cells derived from the renal initial collecting tubules isolated from SV40 large T antigen transgenic rats. Most of the cultured cells were intercalated cells and kallikrein secreting cells were few. (3) Six subjects among the 77 healthy Japanese volunteers have the polymorphism in the renal kallikrein promoter. Urine kallikrein activity was lower and sodium excretion was higher after water loading in the subjects with the promoter polymorphism than the subjects without the polymorphism.