Treatment of diabetic neuropathy by DRG-targeting vector

我们尝试利用能够特异性结合背根神经节神经元的3种7个氨基酸序列（DRG1、DRG2、DRG3），制作针对背根神经节（DRG）的体内基因治疗载体。将分离得到的3种多肽转化到M13噬菌体的PIII上，得到噬菌体载体体系。将绿色荧光蛋白（GFP）引入载体的多克隆位点制备测试载体，观察基因表达效果。将载体注入小鼠蛛网膜下腔，检测gfp在DRG组织中的表达。与使用gst融合蛋白的研究结果相比，通过基因转移在噬菌体载体系统中表达GFP的结果非常低。这可能是由于噬菌体本身的不稳定性造成的，我们需要进一步的安排来提高表达效率。

We have tried to make in vivo gene therapy vectors targeting dorsal root ganglia (DRG) by using 3 kinds of 7 amino acids sequence that could specifically bind to DRG neurons (DRG1, DRG2, DRG3). Phage vector system was obtained by transformations of the isolated 3 kinds of peptites to the PIII of the M13 phage. The test vectors were made by an introduction of green fluorescent protein (GFP) to multi-cloning site of the vector to see the effect of the gene expression. The vectors were administrated to subarachnoid space of mice, and the GFP-expression was examined in DRG tissues. Compared with the results of a study using GST-fusion protein, the expression of GFP by a gene transfer was very low by phage vector system. This might be caused by the unstableness of the phage itself, and we need a further arrangement for the improvement of the expression efficiency.