Molecular biological study on macrophage antimicrobial mechanism based on phospholipase A_2

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We investigated macrophage (MΦ) antimicrobial activity against mycobacteria based on phospholipase A_2 (PLA_2) using molecular biological techniques. (1) Among PLA_2 isotype inhibitors, cytosolic PLA_2 (cPLA_2) inhibitor mildly reduced MΦ anti-mycobacteria activity. (2) MΦ expression of cPLA_2 and secretoryPLA_2-V mRNAs were up-regulated after infection with mycobacteria. (3) cPLA_2 potentiated M Φ anti-mycobacteria activity coupled with apoptosis in the early stage. (4) GFP-tagged cPLA_2 translocated to the membrane of MΦ or CHO cells. Overall, it can be concluded that the cPLA_2-mediated antimicrobial mechanism contributes to MΦ's bactericidal and bacteriostatic function against mycobacterial pathogens but such cPLA_2-dependent mechanism does not play a central role in the MΦ antimycobacterial function.