Reproduction islet transplantation for youth onset I type diabetes The use of the tissue engineering

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After reaching confluency on temperature-responsive culture dishes, cultured rat islet cells were harvested as a uniformly connected tissue sheet by lowering culture temperature. Histological examination showed that harvested cell sheet had a monolayered 2-D structure, which established and retained its structural cell-to-cell connections. Immunohistological staining revealed that islet cell sheet comprised of insulin- and glucagon- staining positive cells. By transplanting islet cell sheets into the subcutaneous space of mice, islet tissues were successfully engineered and sustained. Therapeutic effectiveness of this islet bioengineering procedure was also confirmed in the transplantation study to induced diabetic mice.