Reproduction islet transplantation for youth onset I type diabetes The use of the tissue engineering

生殖胰岛移植在青年发病的I型糖尿病中的应用

• 批准号: 20591227
• 负责人: YAMASHITA Michitoshi
• 金额: $ 2.91万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591227/
• 关键词: I型糖尿病; 膵島移植; 膵島再生; 細胞シート; ティッシュエンジニアリング; 型糖尿病; 再生膵島

After reaching confluency on temperature-responsive culture dishes, cultured rat islet cells were harvested as a uniformly connected tissue sheet by lowering culture temperature. Histological examination showed that harvested cell sheet had a monolayered 2-D structure, which established and retained its structural cell-to-cell connections. Immunohistological staining revealed that islet cell sheet comprised of insulin- and glucagon- staining positive cells. By transplanting islet cell sheets into the subcutaneous space of mice, islet tissues were successfully engineered and sustained. Therapeutic effectiveness of this islet bioengineering procedure was also confirmed in the transplantation study to induced diabetic mice.

在温度反应培养皿上达到汇合后，通过降低培养温度将培养的大鼠胰岛细胞收获为均匀连接的组织片。组织学检查显示收获的细胞片具有单层二维结构，其建立并保留了其结构性细胞与细胞的连接。免疫组化染色显示胰岛细胞片由胰岛素和胰高血糖素染色阳性细胞组成。通过将胰岛细胞片移植到小鼠皮下，成功地构建了胰岛组织。这种胰岛生物工程程序的治疗效果也在诱导糖尿病小鼠的移植研究中得到证实。

项目成果

Morphological and molecular-biological assessment of rat islets pretreated with Mitomycin-C (MMC) before transplantation

移植前用丝裂霉素-C (MMC) 预处理的大鼠胰岛的形态学和分子生物学评估

• 发表时间: 2008
• 作者: Saito Takaharu;Saito Takuro;Sato Y;Ise K;Gunji T;Oshibe I,(Yamamoto M;Waguri S);Gotoh M
• 通讯作者: Gotoh M

Actions of the Japanese Pancreas and Islet Transplantation Association Regarding Transplanted Human Islets Isolated Using Liberase HI

• DOI: 10.1016/j.transproceed.2010.09.142
• 发表时间: 2010-12-01
• 期刊: TRANSPLANTATION PROCEEDINGS
• 影响因子: 0.9
• 作者: Saito, T.;Anazawa, T.;Teraoka, S.
• 通讯作者: Teraoka, S.

膵島細胞による単層細胞シート作製と、膵島組織工学への展開

利用胰岛细胞制备单层细胞片及其在胰岛组织工程中的应用

• 发表时间: 2010
• 作者: 齋藤敬弘;大橋一夫;清水裕史;鵜頭理恵;伊勢一哉;岡野光夫;後藤満一
• 通讯作者: 後藤満一

Islet Transplantation Using Donors After Cardiac Death: Report of the Japan Islet Transplantation Registry

• DOI: 10.1097/tp.0b013e3181ecb044
• 发表时间: 2010-10-15
• 期刊: TRANSPLANTATION
• 影响因子: 6.2
• 作者: Saito, Takuro;Gotoh, Mitsukazu;Teraoka, Satoshi
• 通讯作者: Teraoka, Satoshi

Adenine nucleotide levels in closed automated enzymatic digestion systern for porcine islet isolation : a new aspect of dilution timing.

用于猪胰岛分离的封闭式自动酶消化系统中的腺嘌呤核苷酸水平：稀释计时的新方面。

• 发表时间: 2008
• 作者: Narumi S;Murota K;Asakura Y;Amano N;Ishii T;Adachi M;Hasegawa T;Oshibe I
• 通讯作者: Oshibe I