Regenerative pancreatic islet transplantation for the young onset Type I diabetes mellitus. It is allotransplantation of the pancreatic islet cell sheet.

再生胰岛移植治疗年轻发病的 I 型糖尿病。

• 批准号: 23591507
• 负责人: YAMASHITA Michitoshi
• 金额: $ 3.16万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591507/
• 关键词: 細胞シート; I型糖尿病; 膵島移植; 膵島再生; ティッシュエンジニアリング; 同種移植; 組織工学

Our previous study has demonstrated therapeutic effect of bioengineered islet cell sheets as a novel approach of islet transplantation for diabetes mellitus. Dispersed islet cells obtained from BALB/c mice were then plated on the laminin-5-PIPAAm dishes. After 2 days of culturing, islet cells reached confluent and were harvested as uniformly spread islet cell sheets by lowering culture temperature from 37 degrees C to 20 degrees C for 20 min. Harvested islet cell sheets were transplanted into subcutaneous site of diabetic B6 mice. Normal glucose levels were observed after 3 days of transplantation. Histological examination revealed that the transplanted islet cell sheets with MMC formed clusters of islet tissues and effect to control of inflammatory cell infiltration. It was showed that pretreatment of highly immunogenic crude pancreatic islet sheet with MMC improved their survival in allografts transplantation model.

我们先前的研究已经证明，生物工程胰岛细胞片是一种治疗糖尿病的新方法。将BALB/c小鼠分散的胰岛细胞接种于层粘连蛋白-5-PIPAAm培养皿中。培养2天后，胰岛细胞达到融合，通过将培养温度从37℃降至20℃，20min，收获均匀铺展的胰岛细胞片。将收获的胰岛细胞片移植到B6糖尿病小鼠的皮下。移植后3天观察血糖水平正常。组织学检查显示，植入MMC的胰岛细胞片可形成成簇的胰岛组织，对炎性细胞的侵袭有控制作用。结果表明，在同种异体移植模型中，高免疫原性粗制胰岛片经丝裂霉素C预处理可提高其存活率。

项目成果

Long-term outcome of clinical islet transplantation using donors after cardiac death : a multicenter experience in Japan

心脏死亡后使用供体进行临床胰岛移植的长期结果：日本的多中心经验

• 发表时间: 2013
• 作者: Anazawa T;Kenjo A;Kimura T;Ise K;Haga J;Sato N;Tsuchiya T;Sato T;Gotoh M.
• 通讯作者: Gotoh M.

再生医学研究が変える膵島移植医療の近未来

再生医学研究将改变胰岛移植医学的不久的将来

• 发表时间: 2013
• 作者: Junichiro Haga;Takayuki Anazawa;穴澤 貴行
• 通讯作者: 穴澤 貴行

膵島移植の新たな展開-膵島細胞シートの開発-

胰岛移植的新进展 - 胰岛细胞片的开发 -

• 发表时间: 2011
• 作者: 島田英昭;山崎有浩;谷島聡;大嶋陽幸;小池淳一;清水裕史,大橋一夫,齋藤敬弘,鵜頭理恵,伊勢一哉,大和雅之,岡野光夫,後藤満一
• 通讯作者: 清水裕史,大橋一夫,齋藤敬弘,鵜頭理恵,伊勢一哉,大和雅之,岡野光夫,後藤満一

再生医療叢書5,代謝系臓器

再生医学系列5，代谢系统器官

• 发表时间: 2012
• 作者: Asai H;Kakita H;Aoyama M;Nagaya Y;Asai K .;伊勢一哉
• 通讯作者: 伊勢一哉

Mitomycin-C treatment protects islets from the progression of central damage during culture.

丝裂霉素-C 治疗可保护胰岛在培养过程中免受中央损伤的进展。

• 发表时间: 2011
• 作者: Haga J;Saito T;Anazawa T;Tsuchiya T;Kenjo A;Sato S;Sato N;Saito T;Ise K;Yamamoto M;Waguri S;Gotoh M
• 通讯作者: Gotoh M