Regenerative pancreatic islet transplantation for the young onset Type I diabetes mellitus. It is allotransplantation of the pancreatic islet cell sheet.

我们先前的研究已经证明，生物工程胰岛细胞片是一种治疗糖尿病的新方法。将BALB/c小鼠分散的胰岛细胞接种于层粘连蛋白-5-PIPAAm培养皿中。培养2天后，胰岛细胞达到融合，通过将培养温度从37℃降至20℃，20min，收获均匀铺展的胰岛细胞片。将收获的胰岛细胞片移植到B6糖尿病小鼠的皮下。移植后3天观察血糖水平正常。组织学检查显示，植入MMC的胰岛细胞片可形成成簇的胰岛组织，对炎性细胞的侵袭有控制作用。结果表明，在同种异体移植模型中，高免疫原性粗制胰岛片经丝裂霉素C预处理可提高其存活率。

Our previous study has demonstrated therapeutic effect of bioengineered islet cell sheets as a novel approach of islet transplantation for diabetes mellitus. Dispersed islet cells obtained from BALB/c mice were then plated on the laminin-5-PIPAAm dishes. After 2 days of culturing, islet cells reached confluent and were harvested as uniformly spread islet cell sheets by lowering culture temperature from 37 degrees C to 20 degrees C for 20 min. Harvested islet cell sheets were transplanted into subcutaneous site of diabetic B6 mice. Normal glucose levels were observed after 3 days of transplantation. Histological examination revealed that the transplanted islet cell sheets with MMC formed clusters of islet tissues and effect to control of inflammatory cell infiltration. It was showed that pretreatment of highly immunogenic crude pancreatic islet sheet with MMC improved their survival in allografts transplantation model.