Identification of novel targets related to induce expression of neurotrophic factors at antidepressant in glia

我们试图在神经胶质细胞中找到不依赖单胺的抗抑郁药的新靶点。我们揭示了从抗抑郁药物到神经营养因子表达的途径如下：抗抑郁→MMP激活→FGF2脱落→FGFR1、2→ERK→CREB→神经营养因子表达。这一发现表明，与MMP和FGFR激活相关的分子可能是新靶点的候选分子。另一方面，我们合成了生物素化抗抑郁药配体，并利用该配体分离出抗抑郁药结合蛋白。一些波段已被识别，正在重新确认。

We have tried to identify the monoamine-independent novel target to antidepressant in glia. We revealed a pathway from antidepressant to expression of neurotrophic factors as follows; antidepressant→MMP activation→FGF2 shedding→FGFR1,2→ERK→CREB→expression of neurotrophic factors. This finding suggests that molecules related to activation of MMP and FGFR might be candidates of the novel targets. On the other hand, we synthesized the ligand of biotinylated antidepressant and separated the antidepressant-binding protein by using the ligand. Some bands were identified, which is being reconfirmed.