Development of hybrit mouse with humanized liver using superfreezing preserved human small hepatocytes.

使用超冷冻保存的人类小肝细胞开发具有人源化肝脏的混合小鼠。

• 批准号: 20591615
• 负责人: MIZUGUCHI Toru
• 金额: $ 2.91万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591615/
• 关键词: 自己組織化; 生体機能利用; 移植・再生医療; 応用動物; 再生医学

It is very important to perform surgical operation to obtain cell donation for cell transplantation therapy. Conventional hepatectomy has been considered to be difficult to decrease high mobidity with mortlity. A saline-linked electiric cautery was very effective to achieve vessel sealing and decrease bleeding. In addition, recovery of postoperative Apo-B in this group was much faster than conventional hepatectomy (Hepatogastroenterology 2008 ; 55 : 2188-2192.). Gene transduction of Pdx-1 could transform small hepatocyte into insulin, glucagon, and somatostatine producing cells. Furthermore, small hepatocytes tend to be trans-differentiated into beta cells and mature hepatocytes tend to be trans-differentiated into alpha cells after transduction of Pdx-1 gene (J Hepatobiliary Pancreat Surg 2008 ; 15 : 403-409.). On the other hand, urinary trypsin inhibitor (UTI) is one of the proteins which the liver could produce by itself exclusively. The liver regeneration has been impaired in UTI deficient animals (Liver Int 2009 ; 29 : 979-987.). The gene expression could be depending on host conditions and human races. So, we compared gene expressions using micro DNA array analysis among Japanese patients and Finn patients (Genes Chromosomes Cancer 2010; 49: 28-39.). We proved all above research results and presented the data in both national and international congresses. We also discuss each other to promote further collaboration in future.

进行外科手术以获得细胞捐赠对于细胞移植治疗非常重要。传统的肝切除术被认为难以降低高发病率和死亡率。盐水连接的电烧灼对于实现血管封闭和减少出血非常有效。此外，该组术后Apo-B的恢复比传统肝切除术快得多(Hepatogastroenterology 2008；55：2188-2192.)。 Pdx-1的基因转导可以将小肝细胞转化为产生胰岛素、胰高血糖素和生长抑素的细胞。此外，在转导Pdx-1基因后，小肝细胞倾向于转分化为β细胞，成熟肝细胞倾向于转分化为α细胞(J Hepatobiliary Pancreat Surg 2008；15：403-409.)。另一方面，尿胰蛋白酶抑制剂（UTI）是肝脏只能自行产生的蛋白质之一。 UTI缺陷动物的肝脏再生受到损害(Liver Int 2009；29：979-987.)。基因表达可能取决于宿主条件和人类种族。因此，我们使用微 DNA 阵列分析比较了日本患者和芬兰患者的基因表达（Genes Chromosomes Cancer 2010；49：28-39）。我们证明了所有上述研究成果，并在国内和国际会议上展示了数据。我们还互相讨论以促进未来进一步的合作。

项目成果

Impaired liver regeneration with humoral and genetic disturbances in urinary trypsin inhibitor-deficient mice

尿胰蛋白酶抑制剂缺陷小鼠的体液和遗传紊乱导致肝再生受损

• 发表时间: 2009
• 期刊: Liver Int (in press)
• 作者: Mizuguchi T;Nagayama M;Meguro M;Shibata T;Kaji S;Nobuoka T;Kimura Y;Furuhata T;Hirata K
• 通讯作者: Hirata K

Clinical compliance with an oral uracil/tegafur (UFT) plus leucovorin (LV) regimen as adjuvant chemotherapy in Japanese colorectal cancer patients

• DOI: 10.1007/s10147-009-0888-1
• 发表时间: 2009-10-01
• 期刊: INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
• 影响因子: 3.3
• 作者: Meguro, Makoto;Furuhata, Tomohisa;Hirata, Koichi
• 通讯作者: Hirata, Koichi

Proliferation of Hepatocyte Progenitor Cells Isolated from Adult Human Livers in Serum-Free Medium

• DOI: 10.3727/096368908787236666
• 发表时间: 2008-10
• 期刊: Cell Transplantation
• 影响因子: 3.3
• 作者: K. Sasaki;J. Kon;T. Mizuguchi;Qijie Chen;Hidekazu Ooe;H. Oshima;K. Hirata;T. Mitaka

最善の肝切除術を目指した基本技術と新しい工夫

旨在实现最佳肝切除手术的基本技术和新理念

• 发表时间: 2010
• 作者: 水口徹;他
• 通讯作者: 他

Hirata KCluster analysis of liver functional indicators and ALB-BTR classification to predict postoperative prognosis in 165 HCC patients.

Hirata KCluster 分析肝功能指标和 ALB-BTR 分级预测 165 例 HCC 患者术后预后。

• 发表时间: 2011
• 作者: Mizuguchi T;Kawamoto K;Meguro M;Nakamura Y;Harada K;Nobuoka T
• 通讯作者: Nobuoka T