Effects of bFGF-incorporated gelatin hydrogel and bone marrow-derived Schwann cell transplantation after spinal cord contusion injury in rats

掺有bFGF的明胶水凝胶和骨髓雪旺细胞移植对大鼠脊髓挫伤后的影响

• 批准号: 20591736
• 负责人: OKAWA Akihiko
• 金额: $ 3.08万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591736/
• 关键词: spinal cord injury; gelatin hydrogel; bFGF; allodynia; BBB score; BDA; 脊髄損傷; ゼラチンゲル; 骨髄間質細胞; 神経幹細胞; BBBscore; シュワン細胞; ラット; 徐放

We investigated the effects of a sustained-release system of bFGF from gelatin hydrogel in a rat spinal cord contusion model.One week after spinal cord contusion, gelatin hydrogel (GH) containing bFGF (20μg) was injected into the lesion epicenter of rats. Locomotor recovery was assessed over nine weeks by BBB testing. Sensory abnormalities in the hind paws of all the rats were evaluated at five, seven, and nine weeks.Although BBB scores were better in the bFGF-GH and GH groups than in the saline group, the differences did not reach statistical significance. The bFGF-GH group showed significantly less sensory abnormalities than the saline group at seven weeks.Our study findings suggest that bFGF-incorporated gelatin hydrogel could have therapeutic potential for alleviating mechanical allodynia following spinal cord injury.

在大鼠脊髓挫伤模型上，将含bFGF（20μg）的明胶水凝胶（gelatin hydrogel，GH）注射于损伤中心，观察bFGF缓释系统对脊髓挫伤的治疗作用。通过BBB测试在9周内评估运动恢复。在第5、7和9周时，对所有大鼠后爪的感觉异常进行了评价，虽然bFGF-GH和GH组的BBB评分优于盐水组，但差异未达到统计学显著性。7周时，bFGF-GH组的感觉异常明显少于生理盐水组。我们的研究结果表明，bFGF掺入的明胶水凝胶可能具有减轻脊髓损伤后机械性异常性疼痛的治疗潜力。

项目成果

EFFECTS OF BFGF INCORPORATED GELATIN HYDROGEL AND BONE MARROW STROMAL CELLDERIVED NEURAL PROGENITOR CELL TRANSPLANTATION IN A RAT SPINAL CORD CONTUSION MODEL

BFGF 掺明胶水凝胶和骨髓基质细胞源性神经祖细胞移植对大鼠脊髓挫伤模型的影响

• 发表时间: 2009
• 作者: Hashimoto M.;Furuya T.;Koda M.;Murata A.;Okawa A.;Yamazaki M.;Dezawa M.;Matsuse D.;Tabata Y.;Takahashi K.
• 通讯作者: Takahashi K.

Treatment of rat spinal cord injury with a Rho-kinase inhibitor 3 and bone marrow stromal cell transplantation

Rho激酶抑制剂3联合骨髓基质细胞移植治疗大鼠脊髓损伤

• 发表时间: 2009
• 期刊: Brain Research 1295
• 作者: 大河昭彦;村田淳;山崎正志
• 通讯作者: 山崎正志

Transplantation of human bone marrow stromal cell-derived Schwann cells reduces cystic cavity and promotes functional recovery after contusion injury of adult rat spinal cord

• DOI: 10.1111/j.1440-1789.2010.01130.x
• 发表时间: 2011-02-01
• 期刊: NEUROPATHOLOGY
• 影响因子: 2.3
• 作者: Kamada, Takahito;Koda, Masao;Yamazaki, Masashi
• 通讯作者: Yamazaki, Masashi

ラット脊髄圧挫損傷モデルにおけるbFGF徐放ゼラチンハイドロゲル移植の検討

bFGF缓释明胶水凝胶植入大鼠脊髓挤压损伤模型的检测

• 发表时间: 2008
• 作者: 古矢丈雄;橋本将行;国府田正雄;村田淳;大河昭彦;山崎正志;出澤真理;松瀬大;田畑泰彦;高橋和久
• 通讯作者: 高橋和久

Transplantation of Bone Marrow Stromal Cell-Derived Schwann Cell Reduces Cystic Cavity, Promotes Axonal……

骨髓基质细胞源雪旺细胞移植减少囊腔，促进轴突……

• 发表时间: 2008
• 期刊: J Neurosurgery Spine 9(6)
• 作者: Someya Y;Koda M;Dezawa M;et al.,
• 通讯作者: et al.,