Gelatin hydrogel as a carrier of FGF during rat mandibular distraction

目的：评价明胶水凝胶体系增强重组人成纤维细胞生长因子-2 （rhFGF-2）诱导的大鼠下颌牵张成骨作用的可行性。材料与方法：对28只雄性Wistar大鼠进行下颌牵张。然后，将大鼠分为5组，分别在分心区注射指定的凝胶混合物：1组，单独注射rhFGF-2 (n = 5)；2组，单纯胶原蛋白组（n = 6）；3组：含rhFGF-2的胶原蛋白（n =6）；第4组，单独使用明胶水凝胶（n = 5）；第5组为含rhFGF-2的明胶水凝胶（n = 6）。结果：外周定量计算机断层扫描分析、von Kossa染色和钙黄蛋白染色显示，明胶水凝胶加rhFGF-2（5组）显著增加了皮质骨矿物质密度，5组的细胞数量明显多于3组，且不透明区出现的频率更高。

Purpose: The aim of the present study was to evaluate the feasibility of a gelatin hydrogel system to enhance recombinant human fibroblast growth factor-2 (rhFGF-2)–induced osteogenic effects during rat mandibular distraction.Materials and Methods: Mandibular distraction was performed in 28 male Wistar rats. Then, the rats were divided into 5 groups in which the designated gel mix was inserted into the distracted area: group 1, rhFGF-2 alone (n = 5); group 2, collagen alone (n = 6); group 3, collagen incorporating rhFGF-2 (n =6); group 4, gelatin hydrogel alone (n = 5); and group 5, gelatin hydrogel incorporating rhFGF-2 (n = 6).Results: Peripheral quantitative computed tomographic analysis, von Kossa staining, and calcein staining showed that using gelatin hydrogel with rhFGF-2 (group 5) significantly increased cortical bone mineraldensity, Group 5 also had a significantly larger number of cells compared with group 3 and radiopaque areas were observed morefrequently.