Gelatin hydrogel as a carrier of FGF during rat mandibular distraction

明胶水凝胶作为大鼠下颌牵张过程中 FGF 的载体

• 批准号: 24792182
• 负责人: KABASAWA Yuji
• 金额: $ 2.75万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24792182/
• 关键词: 口腔外科; 骨延長; ゼラチンハイドロゲル; FGF; 骨形成; 成長因子; bFGF; bFGFの臨床応用; 顎骨延長法

Purpose: The aim of the present study was to evaluate the feasibility of a gelatin hydrogel system to enhance recombinant human fibroblast growth factor-2 (rhFGF-2)–induced osteogenic effects during rat mandibular distraction.Materials and Methods: Mandibular distraction was performed in 28 male Wistar rats. Then, the rats were divided into 5 groups in which the designated gel mix was inserted into the distracted area: group 1, rhFGF-2 alone (n = 5); group 2, collagen alone (n = 6); group 3, collagen incorporating rhFGF-2 (n =6); group 4, gelatin hydrogel alone (n = 5); and group 5, gelatin hydrogel incorporating rhFGF-2 (n = 6).Results: Peripheral quantitative computed tomographic analysis, von Kossa staining, and calcein staining showed that using gelatin hydrogel with rhFGF-2 (group 5) significantly increased cortical bone mineraldensity, Group 5 also had a significantly larger number of cells compared with group 3 and radiopaque areas were observed morefrequently.

目的：本研究的目的是评估明胶水凝胶系统的可行性，以提高重组人成纤维细胞生长因子-2（rhFGF-2）诱导的成骨作用在大鼠下颌骨distraction.Materials和方法：下颌骨牵引进行了28只雄性Wistar大鼠。然后，将大鼠分为5组，其中指定的凝胶混合物插入牵张区域：组1，单独的rhFGF-2（n = 5）;组2，单独的胶原（n = 6）;组3，胶原结合rhFGF-2（n =6）;组4，单独的明胶水凝胶（n = 5）;和组5，明胶水凝胶结合rhFGF-2（n = 6）。外周定量CT分析、von Kossa染色和钙黄绿素染色显示，使用明胶水凝胶和rhFGF-2（组5）显著增加皮质骨密度，与组3相比，组5也有显著更多的细胞，观察到更多的不透射线区域。

项目成果

ラット下顎骨延長におけるbFGF含有gelatin hydrogelの有用性

含 bFGF 的明胶水凝胶在大鼠下颌延长中的作用

• 发表时间: 2014
• 作者: 木村 敦;樺沢勇司;佐藤 昌;高原楠旻;樋口佑輔, 松本佳奈子;田畑泰彦;田村 幸彦;青木和広;大谷啓一;小村 健
• 通讯作者: 小村 健

ラット下顎骨延長モデルを用いたbasic fibroblast growth fatcor含有ゼラチンハイドロゲルの有用性評価

使用大鼠下颌延长模型评价含有碱性成纤维细胞生长因子的明胶水凝胶的有效性

• 作者: 木村 敦;樺沢勇司;佐藤 昌;高原楠旻;樋口佑輔;加藤玄樹;菅森泰隆;高橋真理子;田村幸彦;青木和広;大谷啓一;小村 健
• 通讯作者: 小村 健

Gelatin hydrogel as a carrier of recombinant human fibroblast growth factor-2 during rat mandibular distraction.

明胶水凝胶作为大鼠下颌牵张过程中重组人成纤维细胞生长因子-2的载体。

• DOI: 10.1016/j.joms.2014.03.014
• 发表时间: 2014
• 期刊: J Oral Maxillofac Surg.
• 作者: Kimura A;Kabasawa Y;Tabata Y;Aoki K;Ohya K;Omura K
• 通讯作者: Omura K

Mandibular prognathism treatment in patients with obstructive sleep apnea syndrome(OSAS): two case reports

阻塞性睡眠呼吸暂停综合征（OSAS）患者下颌前突的治疗：两例报告

• 发表时间: 2014
• 作者: Yuhji Kabasawa;Masaru Sato;Namiaki Takahara;Satoko Koeda;Yusuke Higuchi;Kanako Matsumoto and Ken Omura
• 通讯作者: Kanako Matsumoto and Ken Omura

The feasibility of gelatin hydrogel as a carrier of basic fibroblast growth factor for bone formation in rat mandibular distraction

明胶水凝胶作为碱性成纤维细胞生长因子载体促进大鼠下颌骨牵张成骨的可行性

• 发表时间: 2014
• 作者: Kimura A;Kabasawa Y;Sato M;Takahara N;Matsumoto K;Higuchi Y;Tabata Y;Aoki K;Ohya K;Omura K
• 通讯作者: Omura K