Gelatin hydrogel as a carrier of FGF during rat mandibular distraction

点击翻译按钮获取中文摘要

Purpose: The aim of the present study was to evaluate the feasibility of a gelatin hydrogel system to enhance recombinant human fibroblast growth factor-2 (rhFGF-2)–induced osteogenic effects during rat mandibular distraction.Materials and Methods: Mandibular distraction was performed in 28 male Wistar rats. Then, the rats were divided into 5 groups in which the designated gel mix was inserted into the distracted area: group 1, rhFGF-2 alone (n = 5); group 2, collagen alone (n = 6); group 3, collagen incorporating rhFGF-2 (n =6); group 4, gelatin hydrogel alone (n = 5); and group 5, gelatin hydrogel incorporating rhFGF-2 (n = 6).Results: Peripheral quantitative computed tomographic analysis, von Kossa staining, and calcein staining showed that using gelatin hydrogel with rhFGF-2 (group 5) significantly increased cortical bone mineraldensity, Group 5 also had a significantly larger number of cells compared with group 3 and radiopaque areas were observed morefrequently.