The mechanism of the increase of pulmonary endothelial cell permeability induced by the increased sympathetic tone and development of the new therapy for ARDS.

我们采用经内皮fitc标记的白蛋白通透性试验，研究了NPY诱导肺内皮细胞通透性增加的机制。培养板由2个室组成，上室采用多孔膜，下室采用24孔微板。HPAEC （Human Pulmonary Artery Endothelial Cells，人肺动脉内皮细胞）接种于细胞间2W。通过测量fitc标记的白蛋白从上腔到下腔60min的浓度来评估内皮细胞单层的通透性。每个NPY条件下的渗透率以%表示，与100%的对照条件（无NPY）相比。NPY 0、10^<-7>、10^<-6>、10^<-5>、10^<-4>、10^<-3>、10^<-2>、10^<-1>、1、10μg/ml的渗透率分别为100、101、102、169、173、163、172、220、229、226%。这些结果表明，NPY增加HPAEC单层的通透性呈剂量依赖性。

We investigated the mechanism of the increase of pulmonary endothelial cell permeability induced by the NPY, using transendothelial FITC-labeled albumin permeability assay.The incubation culture plates were comprised of 2 chambers, the upper chamber utilized a porous membrane and the lower used a 24-well micro plate. HPAEC (Human Pulmonary Artery Endothelial Cells) were seeded in the intercell for 2W. The permeability of the endothelial cell monolayer was assessed by measuring the concentration of FITC-labeled albumin from the upper to the lower chamber for 60minutes. The permeability at each NPY conditions were expressed as % compared to the control condition (no NPY) at 100%. The permeability at NPY 0, 10^<-7>, 10^<-6>, 10^<-5>, 10^<-4>, 10^<-3>, 10^<-2>, 10^<-1>, 1, 10μg/ml were 100, 101, 102, 169, 173, 163, 172, 220, 229, 226% respectively. These results showed that NPY increased the permeability of the HPAEC monolayer in dose-dependent manner.