Mechanism of permeability increase induced by neuropeptide Y in neurogenic pulmonary edema

神经肽Y诱导神经源性肺水肿通透性增加的机制

• 批准号: 16591532
• 负责人: NISHIWAKI Kimitoshi
• 金额: $ 2.24万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591532/
• 关键词: neurogenic pulmonary edema; pulmonary endothelial cell; pulmonary vascular permeability; cell culture; vagal nerve; anesthetic agent; 神経原生肺水腫; 血管透過性亢進; Dibutyryl cAMP; neuropeptide Y; 部分肺還流標本; ARDS; ALI

1. Establishment of neurogenic pulmonary edema induced by fibrin and thrombin injection to the 4th ventricle in rabbit : It was difficult to establish such model and finally abandoned that project.2. Establishment of partially circulated rabbit lung preparation : This experiment was also stopped, because neurogenic pulmonary edema model in rabbit was failed.3. Establishment of monolayer cell culture of human pulmonary endothelial cells : establishments of monolayer cell culture were succeeded in not only human pulmonary endothelial cells but also human coronary arterial endothelial cells and human aortic endothelial cells.4. We investigated the permeability of monolayer of human pulmonary endothelial cells, using monolayer permeability models of endothelial cells, where a double incubation chamber was used for measurement of FITC-labeled albumin transfer through the monolayer. Isoflurane increased the permeability, but sevoflurane and propofol did not. The permeability increase induced by isoflurane was reversed by H-9 (PKG inhibitor), DY9760e (calmodulin inhibitor) and ML-7 (MLCK inhibitor), meaning that isoflurane increased pulmonary endothelial monolayer permeability through cytoskeleton change mediated by cGMP dependent kinase and myosin contraction mediated by myosin light chain kinase.

1.第四脑室注射纤维蛋白和凝血酶诱导的兔神经源性肺水肿模型的建立：该模型的建立困难，最终放弃了该项目.部分循环兔肺标本的建立：由于神经源性肺水肿模型的建立失败，本实验也停止.人肺血管内皮细胞单层培养的建立：不仅人肺血管内皮细胞单层培养成功，而且人冠状动脉内皮细胞和人主动脉内皮细胞单层培养成功.我们研究了单层人肺内皮细胞的渗透性，使用单层内皮细胞渗透性模型，其中双孵育室用于测量FITC标记的白蛋白通过单层的转移。异氟醚增加渗透性，但七氟醚和丙泊酚没有。PKG抑制剂H-9、钙调蛋白抑制剂DY 9760 e和MLCK抑制剂ML-7可逆转异氟烷引起的肺内皮细胞单层通透性增加，这意味着异氟烷通过cGMP依赖性激酶介导的细胞骨架改变和肌球蛋白轻链激酶介导的肌球蛋白收缩来增加肺内皮细胞单层通透性。

项目成果

Neuropeptide Y enhances permeability across a rat aortic endothelial cell monolaver.

神经肽 Y 增强大鼠主动脉内皮细胞单层的通透性。

• 发表时间: 2004
• 期刊: Am.J.Physiol.Heart Circ.Physiol. 286(3)
• 作者: NAN YS;FENG GG;HOTTA Y;NISHIWAKI K;SHIMADA Y;ISHIKAWA N 他
• 通讯作者: ISHIKAWA N 他

Possible role of neuropeptide Y Y3-receptor subtype in rat aortic endothelial cell proliferation under hypoxia,and its specific signal transduction

神经肽Y Y3受体亚型在缺氧条件下大鼠主动脉内皮细胞增殖中的可能作用及其特异性信号转导

• 发表时间: 2007
• 期刊: Am J Physiol Heart Circ Physiol 293
• 作者: Tomoki Nishiyama;et al.;Zhi-Yong Chen
• 通讯作者: Zhi-Yong Chen

Positive inotropic effect of purified green tea catechin derivative in guinea pig hearts; The measurement of cellular Ca2+ and nitric oxide release.

纯化绿茶儿茶素衍生物对豚鼠心脏的正性肌力作用；

• 发表时间: 2006
• 期刊: European Journal of Pharmacology 552
• 作者: Matsuo_A;Bellier JP;Hisano T;Aimi Y;Yasuhara O;Tooyama I;Saito N;Kimura H.;Yoshihiro Hotta
• 通讯作者: Yoshihiro Hotta

A role for L-glutamete ionotropic receptors in the developments of rat neurogenic pulmonary edema.

L-谷氨酸离子型受体在大鼠神经源性肺水肿发展中的作用。

• 发表时间: 2004
• 期刊: Eur.J.Pharmacol. 499(3)
• 作者: KONDO H;FENG GG;NISHIWAKI K;ISHIKAWA N;他
• 通讯作者: 他

Possible role of neuropeptide YY3-receptor subtype in rat aortic endothelial cell proliferation under hypoxia, and its specific signal transduction

神经肽YY3受体亚型在缺氧条件下大鼠主动脉内皮细胞增殖中的可能作用及其特异性信号转导

• 发表时间: 2007
• 期刊: Am J Physiol Heart Circ Physiol 293
• 作者: Isono S.;Tanaka A.;Ishikawa T.;Tagaito Y.;Nishino T.;ZhiYong Chen
• 通讯作者: ZhiYong Chen