Gene therapy for treatment of pulmonary hypertension using plasmid vector

使用质粒载体治疗肺动脉高压的基因疗法

• 批准号: 20591810
• 负责人: MIZUNO Yusuke
• 金额: $ 3.08万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591810/
• 关键词: 肺高血圧; 遺伝子治療; PACAP; HO-1; プラスミドベクター; レンチウイルスベクター; 肺高血圧症; PACAP/VIP; H0-1; エレクトロポレーション

We found decreased expression of neuropeptide VIP, PACAP and decreased expression of their receptors in lung of monocrotaline-induced pulmonary hypertension in rat. It was assumed that the receptor might be induced to compensate for the decrease of VIP and PACAP. Thus, we hypothesized that induction of PACAP in lung could improve pulmonary hypertension. To examine the hypethesis, we created PACAP encoding plasmid and lentivirus vectors and examined the way to introduce them to the lung.

我们发现，在大鼠肺高压大鼠肺组织中，神经肽VIP、PACAP及其受体的表达降低。推测受体可能被诱导以补偿VIP和PACAP的减少。因此，我们假设在肺中诱导PACAP可以改善肺动脉高压。为了验证这一假设，我们创建了PACAP编码质粒和慢病毒载体，并研究了将它们引入肺部的方法。

项目成果

Attenuation of monocrotaline-induced pulmonary hypertension by heme oxygenase-1 involves Toll-like receptor 4 signaling

血红素加氧酶 1 减弱野百合碱诱导的肺动脉高压涉及 Toll 样受体 4 信号传导

• 发表时间: 2010
• 作者: Hiromasa Kawakami;Yusuke Mizuno;Motokazu Koga;Takahisa Goto
• 通讯作者: Takahisa Goto

Effect of Hemin on Pulmonary Artery Pressure of Monocrotaline-Induced Pulmonary Hypertension in Rats

氯化血红素对野百合碱性肺动脉高压大鼠肺动脉压的影响

• 发表时间: 2009
• 作者: 川上裕理

HO-1 expression attenuates monocrotaline-induced pulmonary hypertension through toll-like receptor 4 signaling

HO-1 表达通过 Toll 样受体 4 信号传导减弱野百合碱诱导的肺动脉高压

• 发表时间: 2011
• 作者: 水野祐介;川上裕理;古賀資和;後藤隆久
• 通讯作者: 後藤隆久

Altered Expression of VIP/VPAC2 in Hypertrophic Right Ventricle Induced with Monocrotaline in Rats

野百合碱诱导大鼠肥厚性右心室中 VIP/VPAC2 表达的改变

• 发表时间: 2009

Altered expression in pituitary adenylate cyclase-activating polypeptide and its receptors in monocrotaline-induced pulmonary hypertension in rats

野百合碱诱导的大鼠肺动脉高压中垂体腺苷酸环化酶激活多肽及其受体表达的改变

• 发表时间: 2011
• 作者: Yusuke Mizuno;Motokazu Koga;Tomoya;Irie;Kakahisa Goto
• 通讯作者: Kakahisa Goto