The study of a new target for the effects of volatile anesthetics by immunoblotting.

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Aims : We examined the effects of different types of anesthetics on PKC-γ knockout mice. And we investigated the dynamics of the kinase in brain cells obtained from mice anesthetized with volatile and i.v anesthetics.Method and Results:We measured the required times for loss of righting reflex (rtfLORR) after administration of isoflurane, sevoflurane, or propofol on PKC-γ knockout mice and compared the times with those of wild-type mice. Volatile anesthetics (isoflurane and sevoflurane) significantly prolonged the rtfLORRs in PKC-γ knockout mice compared to those in wild-type mice. On the other hand, no significant difference was observed between knockout and wild-type mice treated with propofol . We also used immunoblotting to investigate the intracellular distribution of PKC-γ and phosphorylated PKC-γ (p-PKC-γ) in brain cell fractions obtained from wild-type mice during the LORR induced by these anesthetics. PKC-γ was significantly decreased in the synaptic membrane fraction (P2), whereas p-PKC-γ was significantly increased in P2. There was no significant change in the supernatant fraction (S). In propofol-treated mice, PKC-γ and p-PKC-γ showed no significant changes in P2 or S.Conclusion : Our results provide new evidence to support the possibility of the involvement of PKC-γ in the actions of volatile anesthetics.