Development of a new therapy using i-RNA method in a newly created pulmonary hypertension model in mice

在新创建的小鼠肺动脉高压模型中使用 i-RNA 方法开发新疗法

• 批准号: 20591844
• 负责人: UEZONO Shoichi
• 金额: $ 2.58万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591844/
• 关键词: 肺高血圧; マウス; 血管リモデリング; 核内転写因子; 核酸医薬; ラット; モノクロタリン; RNA干渉; 先天性心疾患; モノグロタリン; KLF5; 転写因子

Remodeling of pulmonary vasculature (neointimal formation) is a key pathophysiology of development of pulmonary hypertension. The primary purpose of this research is to establish severe form of pulmonary hypertension model in mice. We observed that three-week-long exposure to hypoxic (FiO2=17%) environment with monocrotaline injection caused severe pulmonary hypertension. Unfortunately, more than 50% of these pulmonary hypertensive mice were too sick to tolerate an experiment of measurement of pulmonary artery pressure, necessitating abandon of this experimental model. Using a large cohort of pregnant women with congenital heart disease, we revealed the presence of pulmonary hypertension is a risk factor for maternal and neonatal poor outcome of pregnancy.

肺血管重构（新生内膜形成）是肺动脉高压发展的关键病理生理机制。本研究的主要目的是建立小鼠重度肺动脉高压模型。我们观察到，长时间暴露于缺氧（FiO2=17%）的环境中，单芥碱注射可引起严重的肺动脉高压。不幸的是，超过50%的肺动脉高压小鼠病情严重，无法承受测量肺动脉压的实验，必须放弃该实验模型。通过对一大批患有先天性心脏病的孕妇进行研究，我们发现肺动脉高压是孕妇和新生儿不良妊娠结局的一个危险因素。

项目成果

A Retrospective 7-Year Single-Center Survey of Adverse Maternal and Neonatal Outcomes for Parturients with Congenital Heart Disease : Mode of Delivery and Anesthetic Management

先天性心脏病产妇孕产妇和新生儿不良结局的 7 年单中心回顾性调查：分娩方式和麻醉管理

• 发表时间: 2011
• 期刊: International journal of bstetric anesthesia
• 作者: Hidano G;Uezono S;Terui K
• 通讯作者: Terui K