Analysis of the sperm maturation mechanism by androgen signaling

雄激素信号传导分析精子成熟机制

• 批准号: 20591903
• 负责人: SUZUKI Kichiya
• 金额: $ 3万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591903/
• 关键词: 精巣上体; 精子成熟; アンドロゲンレセプター; コンディショナルノックアウトマウス; コンデイショナルノックアウトマウス

In order to examine androgen-signaling pathway in the epididymis, we have attempted to establish a conditional knock out mice of the androgen receptor gene in the initial segment of the epididymis. We first generated a transgenic mouse carrying a tamoxifen-regulated Cre recombinase gene driven by the epididymis-specific lipocalin gene promoter. When the mice was crossbred with a ROSA26-EGFP mice to confirm the Cre expression in the epididymis, unexpectedly the EGFP expression was recognized in the cauda epididymis. Although the Cre mice was crossbred with a mice carrying a conditional androgen receptor allele to generate a conditional androgen receptor knock out mice in the epididymis, the mice demonstrated a normal sperm concentration and mobility.

为了研究雄激素信号通路在附睾中的作用，我们试图建立附睾起始段雄激素受体基因的条件敲除小鼠。我们首先培育了一只携带他莫昔芬调控的Cre重组酶基因的转基因小鼠，该基因由附睾特异性脂钙蛋白基因启动子驱动。当将小鼠与ROSA26-EGFP小鼠杂交以确认Cre在附睾中的表达时，意外地在附睾尾中识别出EGFP的表达。尽管Cre小鼠与携带条件雄激素受体等位基因的小鼠杂交，在附睾产生条件雄激素受体敲除小鼠，但小鼠表现出正常的精子浓度和活动性。

项目成果

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The shape of the sperm midpiece in intracytoplasmic morphologically selected sperm injection relates sperm centrosomal function

胞浆内形态选择精子注射中精子中段的形状与精子中心体功能相关

• 发表时间: 2010
• 期刊: J Assist Reprod Genetics
• 作者: Ugajin T;Terada Y;Hasegawa H;Nabeshima H;Suzuki K;Yaegashi N
• 通讯作者: Yaegashi N

Intramanchette transport during primate spermiogenesis: expression of dynein, myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein, and Rab27b in the manchette during human and monkey spermiogenesis

• DOI: 10.1111/j.1745-7262.2008.00392.x
• 发表时间: 2008-07-01
• 期刊: ASIAN JOURNAL OF ANDROLOGY
• 影响因子: 2.9
• 作者: Hayasaka, Shinichi;Terada, Yukihiro;Okamura, Kunihiro
• 通讯作者: Okamura, Kunihiro

International Session, Modulator, Reproduction section

国际会议、调制器、复制部分

• 发表时间: 2008

The Role of Foxa Proteins in the Regulation of Androgen Receptor Activity

• DOI: 10.1007/978-0-387-69179-4_25
• 发表时间: 2009-01-01
• 期刊: ANDROGEN ACTION IN PROSTATE CANCER
• 作者: DeGraff, David J.;Yu, Xiuping;Matusik, Robert J.
• 通讯作者: Matusik, Robert J.