Quantitative detection and identification of a wide range of bacteria and fungi composing vaginal flora without culture.

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There are many species of microbes that can not be cultured in media ; therefore, many infectious diseases have been left without diagnosis. For the identification of infectious agents, novel identification methods based on PCR without culture have been reported in the field of bacteriology. In this study, we developed a novel identification method for fungi. Using these examination techniques for bacteria and fungi, we analyzed vaginal swabs taken from patients with informed consent. The results showed that there were no correlations between the number of Candida in the samples and symptoms or the diagnosis of Candida vaginitis. Moreover, bacterial flora and number of fungi in the vagina were found to fluctuate independently.