Research on regenerative medicine of pediatric airways using new materials as scaffolds and scaffold-free cell sheets.

使用新材料作为支架和无支架细胞片的儿科气道再生医学研究。

• 批准号: 20592087
• 负责人: USUI Noriaki
• 金额: $ 2.91万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592087/
• 关键词: 再生医療; 気管軟骨; Scaffold-free; ポリトリメチレンカーボネイト; Tissue engineering; Cell sheet; 回転培養; Cylindrical cartilage; 細胞接着; 生体吸収性足場; 高分子ポリマー; 細胞シート工学

We first tried to use Poly-tri-methylene-carbonate as new materials for scaffolds in cartilage regeneration, however resulted in failure of regeneration. Then, we developed a novel procedure for fabricating engineered cartilage, which maintained the shape and a proper level of rigidity and flexibility, under in vitro conditions using sheet-based tissue engineering techniques. Auricular chondrocytes were harvested from rabbits and cultivated under high-density conditions to form a chondrocyte sheet. The sheet was looped around a silicon tube and cultivated for 6 weeks. The engineered cylindrical cartilage was sufficiently elastic and stiff to maintain the structure without disruption. Histologically, the construct contained a Safranin-O positive cartilaginous matrix accompanied by the expression of type II collagen. The glycosaminoglycan content increased and reached 72% of the native tracheal cartilage after 6 weeks of cultivation.

我们首次尝试将聚三亚甲基碳酸酯作为软骨再生的新支架材料，但导致再生失败。然后，我们开发了一种新的程序，用于制造工程化软骨，保持形状和适当水平的刚性和灵活性，在体外条件下，使用基于片材的组织工程技术。从兔耳收集软骨细胞，并在高密度条件下培养以形成软骨细胞片。将该薄片环绕在硅管上并培养6周。工程圆柱形软骨有足够的弹性和刚度，以保持结构不被破坏。组织学上，该结构含有番红-O阳性软骨基质，伴随着II型胶原蛋白的表达。培养6周后，气管软骨中糖胺聚糖含量增加，达到天然气管软骨的72%。

项目成果

In vitro construction of scafforld-free cylindrical cartilage using cell sheet-based tissue engineering.

使用基于细胞片的组织工程体外构建无支架圆柱形软骨。

• 发表时间: 2010
• 期刊: Pediatr Surg Int 26
• 作者: Tani G;Usui N;kamiyama M;Oue T;Fukuzawa M.
• 通讯作者: Fukuzawa M.

In vitro construction of scaffold-free cylindrical cartilage using cell sheet-based tissue engineering

• DOI: 10.1007/s00383-009-2543-3
• 发表时间: 2010-02-01
• 期刊: PEDIATRIC SURGERY INTERNATIONAL
• 影响因子: 1.8
• 作者: Tani, Gakuto;Usui, Noriaki;Fukuzawa, Masahiro
• 通讯作者: Fukuzawa, Masahiro

小児外科における再生医療-足場を用いない気管軟骨再生の試みと境界領域としての臓器再建医療-

小儿外科中的再生医学 - 不使用支架和器官重建医学作为边界区域的气管软骨再生的尝试 -

• 发表时间: 2010
• 期刊: 日本周産期・新生児医学会雑誌 46巻
• 作者: 谷岳人;臼井規朗;神山雅史;福澤正洋
• 通讯作者: 福澤正洋

軟骨細胞シートを応用した気管軟骨再生法の開発

开发利用软骨细胞片的气管软骨再生方法

• 发表时间: 2010
• 作者: Gakuto Tani;Noriaki Usui;Masafumi Kamiyama;Takaharu Oue;Masahiro Fukuzawa;Gakuto Tani;谷岳人;谷岳人;谷岳人;谷岳人
• 通讯作者: 谷岳人

軟骨細胞シートを応用したin vitroにおける気管軟骨再生法の開発

软骨细胞片体外气管软骨再生方法的开发

• 发表时间: 2009
• 作者: Gakuto Tani;Noriaki Usui;Masafumi Kamiyama;Takaharu Oue;Masahiro Fukuzawa;Gakuto Tani;谷岳人;谷岳人;谷岳人;谷岳人;谷岳人;谷岳人
• 通讯作者: 谷岳人