Basic research of the antigen delivery system that it aimed at the optimization of the DNA vaccine for cancer prevention

许多口腔黏膜疾病都与人乳头瘤病毒（HPV）有关。最近已经很清楚，需要开发更有效的DNA疫苗递送方法，以提高DNA疫苗的效力。本研究探讨了DNA疫苗预防HPV感染的最佳抗原递送系统。结果表明，PLGA纳米球DNA疫苗免疫外加EP免疫可延缓乳头瘤病毒相关口腔癌的发生。体内电穿孔和PLGA纳米球都是有效的DNA疫苗递送方法，因此，它有助于预防乳头瘤病毒诱导的口腔癌。

Many oral mucous diseases are related to human papillomavirus (HPV). Recently it has become clear that more potent methods for DNA vaccine delivery need to be developed to enhance the efficacy of DNA vaccines. In this study, the best antigen delivery system was examined for the purpose of preventing HPV infection by using the DNA vaccine. The results showed that the immunization with PLGA nanosphere DNA vaccines followed by EP in vivo delayed carcinoma development of papillomavirus-associated oral cancer. Both of in vivo electroporation and PLGA nanosphere are potent methods for DNA vaccine delivery, therefore, it contributes to the papillomavirus-induced oral cancer prevention.