Development osseoinductive nano-textured implant and analysis of its gene network

骨诱导纳米纹理植入物的开发及其基因网络分析

• 批准号: 20592289
• 负责人: SATOH Yoshinori
• 金额: $ 2.41万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592289/
• 关键词: 歯学; ナノ材料; シグナル伝達; 蛋白質; 核酸; Implant; bone formation; Implant surface; bone-implant interface; 骨誘導; チタン; インプラント

A novel nano-surfaced titanium has been generated by irradiated with Femto-sec laser and demonstrate stimulation of proliferation and differentiation of rat bone stroma cells. Numbers of cultured cell were counted and expressions of collagen and osteocalcin were studied in vitro. The surface roughness was increased by laser irradiation. In the culture with nano-surfaced Ti, the numbers of cultured were increased than those of machined surface at 7 day and at 10 day after the culture. The number of osteocalcin-positive cell increased than that of collagen-positive cell by the immunohistochemistry at 10 day after the culture.

用飞秒激光照射产生了一种新型的纳米表面钛，并显示了对大鼠骨髓基质细胞增殖和分化的刺激作用。对体外培养的细胞进行计数，并检测胶原和骨钙素的表达。激光辐照增加了表面粗糙度。在含纳米表面钛的培养中，培养7d和10d的培养细胞数均高于机械加工表面的培养细胞。免疫组织化学显示，培养10d后，骨钙素阳性细胞数多于胶原阳性细胞数。

项目成果

レーザー加工を用いた医療用ナノ表面チタンの骨芽細胞の分化に関する影響

激光加工医用纳米表面钛对成骨细胞分化的影响

• 发表时间: 2010
• 期刊: 日大歯学 84巻
• 作者: 稲毛稔彦,佐藤吉則,稲毛寛彦,藤田哲夫,桑田文幸

The regeneration of bone Via Nanoscopic Poss-collagen Nano-surface Ti Implant for bone

通过纳米级胶原蛋白纳米表面钛骨植入物实现骨再生

• 发表时间: 2010
• 作者: 稲毛稔彦,佐藤吉則,稲毛寛彦,藤田哲夫,桑田文幸;稲毛稔彦;T.Inage
• 通讯作者: T.Inage

インプラント用チタン上で培養した骨芽細胞の分化に関する研究

钛种植体上培养的成骨细胞分化研究

• 发表时间: 2010
• 期刊: 日大歯学
• 作者: 稲毛稔彦,佐藤吉則,稲毛寛彦,藤田哲夫,桑田文幸;稲毛稔彦
• 通讯作者: 稲毛稔彦

Nano-surfaced Biomimetic Ti Implant for Bone

纳米表面仿生钛骨植入物

• 发表时间: 2010
• 作者: T.Inage;H.Inage;T.Fujita;Y.Satoh
• 通讯作者: Y.Satoh

Efficiency of Nano-surfaced Ti Implant for bone formation

纳米表面钛植入物促进骨形成的效率

• 发表时间: 2009
• 作者: T.Inage;H.Inage;T.Fujita;F.Kuwata;Y.Satoh
• 通讯作者: Y.Satoh