Functional analysis of cholesterol synthetic pathway in osteochondrodysplasia of congenital cranio-maxillo-facial anomalies

目的：本研究在无血清细胞培养中，研究C27和C30甾醇对软骨细胞ATDC5细胞生长和分化的影响。材料与方法：采用小鼠软骨细胞ATCD5细胞，研究了胆固醇及其前体（C27和C30固醇）对早期和晚期软骨细胞生长分化的影响。采用RT-PCR方法研究了细胞成软骨分化过程中c14 -去甲基化酶、3-酮还原酶、c4 -去甲基化酶等标记基因的表达情况。结果与讨论：我们利用模拟体内软骨分化过程的ATDC5细胞株，研究了C27和C30甾醇对细胞生长和分化的影响。因此，我们发现外源添加C27甾醇对细胞的生长和分化有多种影响，提示C27甾醇在ATDC5细胞系的生长和分化中具有重要作用。

Purpose : In this study, we have studied the effect of C27 and C30 sterols on growth and differentiation of chondrogenic cell line, ATDC5 cells in serum-free cell culture.Material and Methods : Mouse chondrogenic cell line ATCD5 cells have been used to study the effect of cholesterol and its precursor (C27 sterol and C30 sterol) on the growth and differentiation at early and late stage. The expression of the C14-demethylase, 3-ketoreductase, C4-demethylase, and several marker genes in the process of chondrogenic differentiation of the cells have been studied by RT-PCR analysis.Results and discussion : By using ATDC5 cell line, which mimic in vivo chondrogenic differentiation process, we have studied effect of C27 and C30 sterols on the growth and differentiation of the cells. As a result, we have found that exogenously added C27 sterols has various effect on the growth and differentiation of the cell, suggesting that C27 sterol has an important role in the growth and differentiation of the ATDC5 cell line.