Role of Ten-m/Odz3 in the formation, growth and functional maintenance of mandibular condylar cartilage

下颌髁突被推测是通过覆盖在髁突上的成纤维细胞样细胞向软骨细胞分化而发育的；然而，这一过程背后的发育机制尚未揭示。我们描述了10 -m/Odz3 mRNA的时间和空间表达，通过原位杂交测定1天，1周和3周龄的小鼠下颌髁软骨在早期发育阶段。Ten-m/Odz3在纤维层和增殖成熟软骨细胞层中表达，而在增生性软骨细胞层中未检测到。此外，我们使用小鼠软骨细胞ATDC5细胞评估了Ten-m/Odz3的体外表达。Ten-m/Odz3在间充质细胞向软骨细胞分化的早期表达。这些发现表明，Ten-m/Odz3参与软骨细胞的分化，并在下颌髁软骨发育的早期阶段起调节作用。

The mandibular condyle has been speculated to develop via the differentiation of the fibroblast-like cells covering the condyle into chondrocytes ; however, the developmental mechanisms behind this process have not been revealed. We describe the temporal and spatial expression of Ten-m/Odz3 mRNA as determined by in situ hybridization in mouse mandibular condylar cartilage of 1 day, 1 week, and 3 week of age during the early stages of development. Ten-m/Odz3 was expressed in the fibrous layer and the proliferating and mature chondrocyte layers, but was not detected in the hypertrophic chondrocyte layer. Furthermore, we evaluated the in vitro expression of Ten-m/Odz3 using ATDC5 cells, a mouse chondrogenic cell line. Ten-m/Odz3 was expressed during the early stage of the differentiation of mesenchymal cells into chondrocytes. These findings suggest that Ten-m/Odz3 is involved in the differentiation of chondrocytes and that it acts as a regulatory factor in the early stages of the development of mandibular condylar cartilage.