Basic research on prevention of aspiration pneumonia in acute stroke patients

急性脑卒中患者吸入性肺炎预防的基础研究

• 批准号: 20592556
• 负责人: KAMAKURA Yayoi
• 金额: $ 2.91万
• 依托单位: Aichi Prefectural College of Nursing & Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592556/
• 关键词: 脳卒中急性期; 誤嚥性肺炎; 唾液中sIgA; 唾液中IL-6; 唾液中EGF; 口腔内細菌; ELISA; real-time PCR法; 脳卒中; 唾液中Streptococcus pneumoniae; 唾液中Streptococcus mitis; 唾液中Pseudomonas aeruginosa; 嚥下障害; 不顕性誤嚥; 急性期看護

Acute stroke patients have a high risk of aspiration pneumonia. The purpose of this study was to examine the relationship among complication of aspiration pneumonia and salivary secretory immunoglobulin A (sIgA), interleukin 6 (IL-6), epidermal growth factor (EGF), and oral bacteria. Fourteen stroke patients (Age : mean±SD ; 79.5±9.0) were classified into a non-pneumonia group (6 people) or a pneumonia group (8 people). Saliva samples were collected three times (10am, 14am, and 18 pm) on days 5, 7, 9, 11 and 13 in the hospital. Samples were measured by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (real-time PCR), then compared between the two groups. As a result, there was a significant increase in the salivary sIgA (concentration of sIgA × saliva volume) in the pneumonia group (ANOVA ; p<0.01). The IL-6 and EGF concentrations did not significantly differ between groups, whereas the concentration of EGF correlated with the sIgA level (rs=0.574～0.900). Two people in the non-pneumonia group evidenced in Streptococcus pneumoniae. The pneumonia group people were given antibiotics. Six people showed less Streptococcus pneumoniae and Streptococcus mitis, and 4 people demonstrated increased Pseudomonas aeruginosa.

急性中风患者有较高的吸入性肺炎风险。本研究旨在探讨吸入性肺炎并发症与唾液分泌免疫球蛋白A （sIgA）、白细胞介素6 （IL-6）、表皮生长因子（EGF）及口腔细菌的关系。14例脑卒中患者（年龄：mean±SD； 79.5±9.0）分为非肺炎组（6人）和肺炎组（8人）。在第5天、第7天、第9天、第11天和第13天分别于上午10点、14点和晚上18点采集唾液样本。采用酶联免疫吸附试验（ELISA）和实时聚合酶链反应（real-time PCR）对样品进行检测，并对两组进行比较。结果肺炎组大鼠唾液sIgA （sIgA浓度×唾液体积）显著升高（方差分析p<0.01）。各组间IL-6和EGF浓度差异不显著，而EGF浓度与sIgA水平相关（rs=0.574~0.900）。两名非肺炎组患者肺炎链球菌感染。肺炎组给予抗生素治疗。6例患者肺炎链球菌和支气管炎链球菌减少，4例患者铜绿假单胞菌增多。

项目成果

Effects of secretion of salivary immunoglobulin A on aspiration pneumonia in elderly acute stroke patients.

唾液免疫球蛋白A分泌对老年急性脑卒中患者吸入性肺炎的影响

• 发表时间: 2010
• 作者: 熊澤友紀;鎌倉やよい;米田雅彦;深田順子
• 通讯作者: 深田順子

Aspiration pneumonia complication and change of oral bacteria in acute stroke patients.

急性脑卒中患者吸入性肺炎并发症及口腔细菌变化

• 发表时间: 2011
• 作者: 熊澤友紀;鎌倉やよい;米田雅彦;深田順子
• 通讯作者: 深田順子

脳卒中急性期肺炎合併患者における唾液中のIL-6とEGFの分泌動態

急性脑卒中肺炎患者唾液中IL-6和EGF的分泌动态

• 发表时间: 2010
• 作者: 熊澤友紀;鎌倉やよい;米田雅彦;深田順子;片岡笑美子;波多野範和;長谷川康博
• 通讯作者: 長谷川康博

脳卒中急性期の誤嚥性肺炎予防に関する唾液中sIgAの検討

唾液sIgA检查预防脑卒中急性期吸入性肺炎

• 发表时间: 2009
• 作者: 熊澤友紀;鎌倉やよい;米田雅彦;深田順子;片岡笑美子;波多野範和;長谷川康博
• 通讯作者: 長谷川康博

Aspiration pneumonia complication and change of bacteria in acute stroke patients

急性脑卒中患者吸入性肺炎并发症及细菌变化

• 发表时间: 2011
• 作者: Kumazawa;Y.;Kamakura;Y.;Yomeda;M.;Fukada;J.
• 通讯作者: J.