Development of new tumor vaccines using antigen presentation by recycling MHC class II molecules

通过回收 MHC II 类分子利用抗原呈递开发新的肿瘤疫苗

• 批准号: 20599023
• 负责人: YUSUKE Mimura
• 金额: $ 2.15万
• 依托单位: Department of Clinical Research National Hospital Organization, National Sanyo Hospital
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20599023/
• 关键词: 抗原認識; 抗原提示; MHCクラスII分子; CD4+T細胞; 癌ワクチン; Protein folding; MHC class II molecules; CD4+ T cells; MHC classII molecule

Efficient antigen presentation to CD4+ T cells is essential for activation of anti-tumor immunity. In order to elicit robust T cell responses with smaller doses of antigen, we have designed a new vaccine in which a CD4+ T cell epitope of ovalbumin is inserted in the complementary determining region of an immunoglobulin molecule. This inserted epitope is supposed to be presented to CD4+ T cells after direct binding to a recycling MHC class II molecule on the surface of an antigen presenting cell and processing in the endosome, in contrast to the known processing pathway involving the late endosomes and lysosomes.

有效的抗原呈递到CD4+ T细胞是激活抗肿瘤免疫的必要条件。为了用更小剂量的抗原引起强大的T细胞应答，我们设计了一种新的疫苗，在这种疫苗中，将卵白蛋白的CD4+ T细胞表位插入免疫球蛋白分子的互补决定区。与已知的涉及晚期核内体和溶酶体的加工途径不同，这个插入的表位在直接结合抗原呈递细胞表面的MHC II类再循环分子并在核内体中加工后被呈递给CD4+ T细胞。

项目成果

Prognostic potential of FOXP3 expression in non-small cell lung cancer cells combined with tumor-infiltrating regulatory T cells

• DOI: 10.1016/j.lungcan.2011.06.002
• 发表时间: 2012-01-01
• 期刊: LUNG CANCER
• 影响因子: 5.3
• 作者: Tao, Hiroyuki;Mimura, Yusuke;Ueoka, Hiroshi
• 通讯作者: Ueoka, Hiroshi

High-throughput screening of glycan-binding proteins using miniature pig kidney N-glycan-immorbilized beads

使用微型猪肾 N-聚糖固定珠高通量筛选聚糖结合蛋白

• 发表时间: 2008
• 期刊: Chemistry & Biology 15
• 作者: Kim;Y.-G;Mimura;Y. 他
• 通讯作者: Y. 他

Therapeutic Monoclonal Antibodies_From Bench to Clinic

治疗性单克隆抗体_从实验室到临床

• 发表时间: 2009
• 作者: Mimura;Y;Jefferis;R;Mimura-Kimura;Y;Abrahams;J;Rudd;PM;Yusuke Mimura (分担)
• 通讯作者: Yusuke Mimura (分担)

High-throughput screening of glycan-binding proteins using miniature pig kidney N-glycan-immorbilized beads.

使用微型猪肾 N-聚糖固定化珠对聚糖结合蛋白进行高通量筛选。

• 发表时间: 2008
• 期刊: Chem.Biol. 15
• 作者: Kim;Y-G;Shin;D-S;Yang;Y-H;Gil;G-C;Park;C-G;Mimura;Y;et al.
• 通讯作者: et al.

Induction of anti-tumor immunity via recycling MHC class II-guided processing

通过回收 MHC II 类引导加工诱导抗肿瘤免疫力

• 发表时间: 2008
• 作者: 三村雄輔;他
• 通讯作者: 他