Generation, differentiation and integration of neural stem cells into neural circuits in the adult olfactory system

嗅觉感觉神经元是由神经干细胞（NSCs）终生生成的。神经球测定法是研究正常组织中这些NSCs的标准方法。为了从小鼠嗅上皮中分离NSCs，我们用无血清培养系统从嗅粘膜中制备神经球。然后，我们成功地将这些细胞分化为嗅觉神经元和星形胶质细胞，培养基中不添加有丝分裂原如EGF和FGF。此外，嗅球（OB）中间神经元也在整个生命过程中由NSCs生成。接下来，我们利用慢病毒系统分析了OB中间神经元的感觉输入依赖性发育。我们在新生儿阶段将慢病毒（CMV启动子- gapeyfp基因）注射到SVZ中，随后进行鼻腔阻塞。我们发现气味诱导的活动是OB中间神经元树突伸长和树突树突化的必要条件。

Olfactory sensory neurons are generated from neural stem cells (NSCs) throughout life. Neurosphere assays are standard studying these NSCs in normal tissues. To isolate NSCs) from the mouse olfactory epithelium, we made neurospheres from the olfactory mucosa by a serum-free culture system. Then, we succeeded to differentiate those to olfactory neurons and astrocytes in media, not supplemented with mitogens such as EGF and FGF. Further, olfactory bulb (OB) interneurons are also generated from NSCs throughout the life. Next, by using the lentiviral system, we have analyzed the sensory input-dependent development of OB interneurons. We injected the lentivirrus (CMV promoter-gapEYFP gene) into the SVZ at neonatal stage, followed by naris occlusion.We found that the odorant-induced activity is necessary for the dendrite elongation and dendrite arborization of OB interneurons.