Study of natural inducer for coral larval settlement and metamorphosis.

珊瑚幼虫沉降和变态天然诱导剂的研究。

• 批准号: 20611003
• 负责人: KITAMURA Makoto
• 金额: $ 3.24万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20611003/
• 关键词: 造礁サンゴ; 幼生; 変態誘引物質; 無節サンゴモ; サンゴ幼生; 変態誘因物質; 紅藻類; バクテリア; Luminaolide; 11-Deoxyfistularin-3; 構造活性相関; 複合系化学物質; トゲスギミドリイシ; コユビミドリイシ; ルリサンゴ; サンゴモ(CCA); ll-deoxyfistularin-3

A new metamorphosis-enhancing macrodiolide, luminaolide, was isolated from the crustose coralline algae (CCA) Hydrolithon reinboldii. Its structure was determined by spectroscopic analysis. A fraction (1.30 μg/mL) eluted with 80% aqueous MeOH by ODS gel column chromatography of the same CCA extract induced larval metamorphosis (25.9±7.4%) against Leptastrea purpurea, and its metamorphosis-inducing activity was further enhanced to 92.6±2.9% with the addition of 1 (25.6 ng/mL).Furthermore, eight bromotyrosine derivatives including 11-deoxyfistularine-3 were prepared and tested for their metamorphosis-inducing activity against larvae of scleractinian coral Pseudosiderastrea tayamai. The following structure-activity relationships were noted : (i) two spiroisoxazolin moieties are essential, tyramine derivetive between both spiroisoxazolin moieties is not essential for the activity. (ii) Two hydroxyl groups in each spiroisoxazolin moiety are important for the activity.

从甲壳珊瑚藻Hydrolithon reinboldii中分离到一种新的促进变态的巨噬细胞，即发光藻。通过光谱分析确定了其结构。一小部分经ODS凝胶柱层析，用80%甲醇水溶液洗脱，1.30 μg/mL的CCA提取物诱导幼虫变态（25.9±7.4%），添加1.0mg/L的1.0mg/L此外，制备了包括11-deoxyfistularine-3在内的8种溴酪氨酸衍生物，并测试了它们对石珊瑚Pseudosiderastrea tayamai幼虫的变态诱导活性。注意到以下结构-活性关系：（i）两个螺异恶唑啉部分是必需的，两个螺异恶唑啉部分之间的酪胺衍生物对于活性不是必需的。(ii)每个螺异恶唑啉部分中的两个羟基对于活性是重要的。

项目成果

Regulation of sperm flagellar motility activation and chemotaxis caused by egg-derived substance(s) in sea

海洋中卵源性物质对精子鞭毛运动激活和趋化性的调节

• 发表时间: 2009
• 期刊: Cell Motil. Cyt. 66
• 作者: cucumber;M. Morita;M. Kitamura;A. Nakajima;E. S. Susilo;A. Takemura;M. Okuno
• 通讯作者: M. Okuno

サンゴ幼生に作用する無節サンゴモの他感作用物質(2)

作用于珊瑚幼虫的不分节珊瑚藻的过敏物质 (2)

• 发表时间: 2009
• 作者: Yasuhiro Ohki;Kazunari Yasumura;Masaru Ando;Satoko Shimokata;Kazuyuki Tatsumi;北村誠・Peter J. Schupp・中野義勝・上村大輔
• 通讯作者: 北村誠・Peter J. Schupp・中野義勝・上村大輔

Biologically active marine natural products

具有生物活性的海洋天然产物

• 发表时间: 2009
• 期刊: Heterocycles 78
• 作者: K. Makamura;M. Kitamura;D. Uemura
• 通讯作者: D. Uemura

造礁サンゴ幼生の変態誘因物質

诱导造礁珊瑚幼虫变态的物质

• 发表时间: 2008
• 期刊: 化学と生物 46
• 作者: 守田昌哉;北村誠;中島綾子;Endang S.Susilo;竹村明洋;奥野誠;阿部晃聡,加藤純雄,小笠原正剛,中田真一,若林誉,中原祐之輔;北村誠
• 通讯作者: 北村誠

Regulation of sperm flagellar motility activation and chemotaxis caused by egg-derived substance(s)in sea cucumber

海参卵源性物质对精子鞭毛运动激活和趋化性的调节

• 发表时间: 2009
• 期刊: Cell Motility and the Cytoskeleton 66
• 作者: 守田昌哉;北村誠;中島綾子;Endang S.Susilo;竹村明洋;奥野誠
• 通讯作者: 奥野誠