Mechanisms for methylation imprinting establishment afterfertilization
Mechanisms for methylation imprinting establishment afterfertilization
批准号:
20678002
负责人:
TANIMOTO Keiji
金额:
$66.56万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (S)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2012
中文摘要
基因组印记是哺乳动物中的一种表观遗传现象,其中一个基因子集根据其亲本来源而单等位地表达。为了揭示区分亲本来源及其在H19-ICR序列中的沉积位点的表观遗传标记的身份,采用转基因小鼠方法,并证明了以下结果:1)父源H19-ICR获得甲基化独立于其在精子中的建立2)CTCF位点不需要建立父源等位基因,3)H19-ICR中与卵母细胞中的小RNA序列相对应的序列进行甲基化印迹分析4)H19-ICR的差异甲基化是通过其父亲等位基因特异性甲基化和母亲等位基因特异性保护性抗甲基化来建立的5)H19-ICR中的Oct-sox基序具有保护性抗甲基化,6)鸡HS 4绝缘子不能保护H19-ICR免受差异DNA甲基化的影响。
英文摘要
enomic imprinting is an epigenetic phenomenon in mammals, in which a subset of genes are mono-allelically expressed depending on their parental origin.To reveal identity of the epigenetic signature discriminating the parental origin and its deposition site within the H19-ICR sequence, transgenic mouse methodology was employed and following findings were demonstrated:1) Paternal H19-ICR acquired methylation independently of its establishment in sperm2) CTCF sites were not required to establish paternal-allele-specific methylation in pre-implantation embryos3) Sequences in the H19-ICR corresponding to the small RNA sequences in oocytes were dispensable for methylation imprinting4) Differential methylation of the H19-ICR was established by its paternal-allele-specific methylation and maternal-allele-specific protection-against-methylation5) Oct-sox motif in the H19-ICR harbored protection-against-methylation activity in vivo after implantation6) Chicken HS4 insulator did not protect the H19-ICR from differential DNA methylation.
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H19-ICRの刷り込みメチル化におけるOct motifのin vivo機能解析
H19-ICR 印记甲基化中 Oct 基序的体内功能分析
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[坂口龍太, 岡村永一, 谷本啓司]
通讯作者:
谷本啓司
The chicken HS4 insulator does not protect the H19 ICR from differential methylation in YAC transgenic mice
鸡 HS4 绝缘体不能保护 YAC 转基因小鼠中 H19 ICR 免受差异甲基化的影响
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[岡村永一, 松崎仁美, 牛木亜季, 谷本啓司]
通讯作者:
谷本啓司
Post-fertilization methylation imprinting in the randomly integrated transgenic H19-ICR fragment(poster)
随机整合的转基因 H19-ICR 片段中的受精后甲基化印记(海报)
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[松崎仁美, 谷本啓司]
通讯作者:
谷本啓司
メディカル・サイエンス・インターナショナル遺伝情報の発現制御 (Gene Control)転写機構からエピジェネティクスまで第9章 胚発生における遺伝子調節
Medical Science International 遗传信息的表达控制(基因控制)从转录机制到表观遗传学第9章胚胎发育中的基因调控
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[A. Yamakata, H. Shimizu, M. Osawa, S. Oiki, 谷本啓司]
通讯作者:
谷本啓司
DOI:
10.1073/pnas.0808506106
发表时间:
2008-12-23
期刊:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子:
11.1
作者:
[Hou, Chunhui, Zhao, Hui, Dean, Ann]
通讯作者:
Dean, Ann
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