Evaluation of the Embryo Quality for Elective Single Embryo Transfer

选择性单胚胎移植胚胎质量评价

• 批准号: 20680026
• 负责人: MURAYAMA Yoshinobu
• 金额: $ 16.22万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20680026/
• 关键词: バイオメカニクス; ヒト生殖補助医療; 卵子; 透明帯; 不妊治療; 生体弾性率; マイクロタクタイルセンサ; ガラス化保存; 卵子成熟; ラマン分光法; 複屈折率

To enable improved success rate of IVF, we developed the technology to optimize embryo selection, having the highest implantation potential while ensuring no damage to embryos using zona elasticity as selection criteria. First, "zona hardening" was demonstrated mechanically following fertilization at the PN stage, Young's modulus decreased gradually as the embryo developed. Evaluation of the quality of expanded blastocysts(EPB) shows that the quality of EPBs could also be evaluated from elasticity parameters. Furthermore observations indicated that ZPs of embryos generated in vivo were significantly harder than those of embryos generated in vitro at each stage. We examined the correlations between subsequent ZP elasticity changes after IVF treatment and quality of embryos and the results showed that the pregnancy rate after MTS-eSET was almost the same with that after double-embryo transfer. It was also examined that both theoretically and experimentally the MTS test does not cause any damage to embryos. Furthermore, we developed a cryo-nano-hole vitrification container with a low concentration of CPA solution with no serum and mouse and human blastocysts can be cryopreserved successfully.

为了提高IVF的成功率，我们开发了优化胚胎选择的技术，具有最高的植入潜力，同时使用弹性作为选择标准，确保胚胎不受损伤。第一，在PN阶段受精后机械地表现出“弹性硬化”，杨氏模量随着胚胎的发育而逐渐降低。对扩张囊胚（EPB）质量的评价表明，扩张囊胚的质量也可以通过弹性参数进行评价。此外，观察表明，在体内产生的胚胎的ZPs显着比在体外产生的胚胎在每个阶段更难。我们检查了IVF治疗后随后的ZP弹性变化与胚胎质量之间的相关性，结果显示MTS-eSET后的妊娠率与双胚移植后的妊娠率几乎相同。还研究了MTS测试在理论上和实验上都不会对胚胎造成任何损害。在此基础上，我们研制了一种无血清低浓度CPA溶液的低温纳米孔玻璃化冷冻容器，成功地冷冻了小鼠和人的囊胚。

项目成果

Elasticity Measurement of Zona Pellucida Using a Micro Tactile Sensor to Evaluate Embryo Quality

使用微型触觉传感器测量透明带的弹性来评估胚胎质量

• 发表时间: 2008
• 期刊: Journal of Mammalian Ova Research
• 作者: Y. Murayama;M. Yoshida;J. Mizuno;H. Nakamura;S. Inoue;Y. Watanabe;K. Akaishi;H. Inui;C. E. Constantinou and S. Omata
• 通讯作者: C. E. Constantinou and S. Omata

Pluripotency and Transgenosis of Bovine ES-like Cells

牛 ES 样细胞的多能性和转基因

• 发表时间: 2011
• 作者: 齋藤成夫、村山嘉延;ら
• 通讯作者: ら

A study about the stem cell local injection technology to the embryo

干细胞胚胎局部注射技术的研究

• 发表时间: 2010
• 作者: 高橋春隆、村山嘉延;ら
• 通讯作者: ら

新しい顕微鏡システムを用いた生体組織弾性率分布計測法の定量的検討

利用新型显微镜系统定量研究生物组织弹性模量分布测量方法

• 发表时间: 2009
• 作者: 永井千晴、村山嘉延;ほか
• 通讯作者: ほか

Variations in local elastic modulus along the length of the aorta as observed by use of a scanning haptic microscope (SHM)

• DOI: 10.1007/s10047-011-0596-2
• 发表时间: 2011-12-01
• 期刊: JOURNAL OF ARTIFICIAL ORGANS
• 影响因子: 1.3
• 作者: Moriwaki, Takeshi;Oie, Tomonori;Nakayama, Yasuhide
• 通讯作者: Nakayama, Yasuhide