Evaluation of the Embryo Quality for Elective Single Embryo Transfer

为了提高IVF的成功率，我们开发了优化胚胎选择的技术，具有最高的植入潜力，同时使用弹性作为选择标准，确保胚胎不受损伤。第一，在PN阶段受精后机械地表现出“弹性硬化”，杨氏模量随着胚胎的发育而逐渐降低。对扩张囊胚（EPB）质量的评价表明，扩张囊胚的质量也可以通过弹性参数进行评价。此外，观察表明，在体内产生的胚胎的ZPs显着比在体外产生的胚胎在每个阶段更难。我们检查了IVF治疗后随后的ZP弹性变化与胚胎质量之间的相关性，结果表明MTS-eSET后的妊娠率与双胚移植后的妊娠率几乎相同。还研究了MTS测试在理论上和实验上都不会对胚胎造成任何损害。在此基础上，我们研制了一种无血清低浓度CPA溶液的低温纳米孔玻璃化冷冻容器，成功地冷冻了小鼠和人的囊胚。

To enable improved success rate of IVF, we developed the technology to optimize embryo selection, having the highest implantation potential while ensuring no damage to embryos using zona elasticity as selection criteria. First, "zona hardening" was demonstrated mechanically following fertilization at the PN stage, Young's modulus decreased gradually as the embryo developed. Evaluation of the quality of expanded blastocysts(EPB) shows that the quality of EPBs could also be evaluated from elasticity parameters. Furthermore observations indicated that ZPs of embryos generated in vivo were significantly harder than those of embryos generated in vitro at each stage. We examined the correlations between subsequent ZP elasticity changes after IVF treatment and quality of embryos and the results showed that the pregnancy rate after MTS-eSET was almost the same with that after double-embryo transfer. It was also examined that both theoretically and experimentally the MTS test does not cause any damage to embryos. Furthermore, we developed a cryo-nano-hole vitrification container with a low concentration of CPA solution with no serum and mouse and human blastocysts can be cryopreserved successfully.