Analysis of cortico-basal ganglia loop circuits with novel retrograde transneuronal tracing technique

应用狂犬病毒逆行跨神经元标记技术，分析了猕猴皮质-基底节环路的结构基础。我们证明了存在一个封闭的，平行的循环涉及背侧纹状体和一个开放的循环涉及腹侧纹状体。此外，我们还克隆了狂犬病病毒CVS株的全长基因组和全部基因，并建立了狂犬病病毒cDNA质粒拯救系统。这使我们能够实现多色逆行跨神经元示踪标记蛋白，如GFP和RFP。

By means of retrograde transneuronal labeling technique with rabies virus, the structural basis for cortico-basal ganglia loop circuits was analyzed in macaque monkeys. We demonstrated the existence of a closed, parallel loop involving the dorsal striatum and an open loop involving the ventral striatum. Moreover, we performed cloning of full-length genome and all genes of the CVS strain of rabies virus, and established the rescue system for a rabies virus vector from cDNA plasmid. This enables us to achieve multi-color retrograde transneuronal tracing with maker proteins such as GFP and RFP.