Regulation of brain function by thermo-sensor

通过热传感器调节大脑功能

• 批准号: 20700309
• 负责人: SHIBASAKI Koji
• 金额: $ 2.83万
• 依托单位: National Institutes of Natural Sciences Okazaki Research Facilities
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20700309/
• 关键词: TRPV4; 海馬; 体温; 脳内温度; 神経興奮; てんかん; 産学官; 特許; 温度センサー; リアルタイム測定; 温度プローブ; 睡眠-覚醒

I found hippocampus strongly expressed TRPV4 in addition to choloid plexus, where TRPV4 expression was been reported. I considered that body temperature activates brain TRPV4, and the activation might contribute to depolarization of the resting membrane potential (RMP). I compared the RMP between wild-type and TRPV4-deficient neurons at 37℃, and found the wild-type RMP was approximately 5 mV more depolarized than the TRPV4-deficient RMP. I also performed current-injection experiments in both neurons, and found that TRPV4-deficient neurons required much bigger currents to get their firing. Furthermore, I found that glutamate-induced Ca^<2+> influx in wild-type neurons was much bigger than that in TRPV4-deficient neurons at 37℃. APV (a blocker of NMDA receptors) inhibited the enhancement of glutamate-induced Ca^<2+> influx in wild-type neurons at 37℃, suggesting that body temperature depolarized RMP near the thresholds for NMDA receptor activation compared with room temperature condition. Therefore, I conclude that TRPV4 is activated by body temperature in hippocampus, and produces proper environments for their firing.

我发现，除了已报道TRPV4表达的胆丛外，海马也强烈表达TRPV4。我认为体温激活脑TRPV4，这种激活可能有助于静息膜电位（RMP）的去极化。我比较了野生型和TRPV4缺陷型神经元在37℃下的RMP，发现野生型RMP比TRPV4缺陷型RMP去极化约5 mV。我还在这两种神经元中进行了电流注入实验，发现TRPV4缺陷的神经元需要更大的电流才能激活。此外，我还发现在37℃下，谷氨酸诱导的野生型神经元中的Ca^<2+>内流比TRPV 4缺陷型神经元中的大得多。APV（NMDA受体阻断剂）可抑制37℃下谷氨酸诱导的野生型神经元Ca^2+内流的增加，表明与室温条件相比，体温可使RMP在NMDA受体激活阈值附近去极化。因此，我的结论是TRPV4在海马中被体温激活，并为它们的放电创造适当的环境。

项目成果

膜伸展刺激活性化チャネルを介した軸索伸長の制御:新規ポジティブフィードバック機構

通过膜拉伸刺激激活通道控制轴突生长：一种新颖的正反馈机制

• 发表时间: 2009
• 作者: 柴崎貢志;小野勝彦;富永真琴;石崎泰樹;Koji Shibasaki;柴崎貢志;柴崎貢志
• 通讯作者: 柴崎貢志

Thermo TRP channels, TRPV2 and TRPA1 regulate axon outgrowth in developing dorsal root ganglion and motor neurons.

Thermo TRP 通道、TRPV2 和 TRPA1 调节发育中的背根神经节和运动神经元的轴突生长。

• 发表时间: 2008
• 作者: Koji Shibasaki;Namie Murayama;Makoto Tominaga
• 通讯作者: Makoto Tominaga

神経系のTRPチャネルと体温

神经系统 TRP 通道和体温

• 发表时间: 2008
• 期刊: Clinical Neuroscience 26(7)
• 作者: 柴崎貢志;富永真琴
• 通讯作者: 富永真琴

Thermosensitive TRP channels, TRPV2 and TRPA1 regulate axon outgrowth in developing dorsal root ganglion and motor neurons.

热敏 TRP 通道、TRPV2 和 TRPA1 调节发育中的背根神经节和运动神经元的轴突生长。

• 发表时间: 2008
• 作者: Shibasaki K;Murayama N;Tominaga M
• 通讯作者: Tominaga M

The TRPV4 Cation Channel Mediates Stretch-evoked Ca2+ Influx and ATP Release in Primary Urothelial Cell Cultures

• DOI: 10.1074/jbc.m109.020206
• 发表时间: 2009-08-07
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Mochizuki, Tsutomu;Sokabe, Takaaki;Tominaga, Makoto
• 通讯作者: Tominaga, Makoto