Interaction between brain temperature and neuronal excitability

生理脑温度是神经元功能的重要决定因素，并且已经确定温度的变化对海马神经元活动具有动态影响。我以前发现，热传感器TRPV4（在34°C以上激活）被海马神经元中的生理大脑温度激活，从而控制它们的体外兴奋性。因此，可以认为温度传感器TRPV4可以将温度信息转换为哺乳动物大脑中的电兴奋性。我研究了TRPV4是否通过体内脑温激活来调节神经元的兴奋性。在自由活动状态下记录野生型和TRPV4KO小鼠的脑电图（EEG），结果显示TRPV4KO小鼠海马活动较野生型小鼠明显减弱。与EEG数据一致，TRPV 4 KO海马神经元在生理温度下表现出异常的兴奋性突触后电位（EPSP）。综上所述，我们首次揭示了TRPV4是哺乳动物脑温度到神经元兴奋性的重要翻译者。

Physiological brain temperature is an important determinant for neuronal functions, and it is well established that changes in temperature have dynamic influences on hippocampal neuronal activities. I previously revealed that a thermo-sensor TRPV4 (activated above 34°C) is activated by physiological brain temperature in hippocampal neurons and thereby controls their excitability in vitro. Therefore, it is considered that the thermo-sensor TRPV4 can convert temperature information to electrical excitability in mammalian brains. I examined whether TRPV4 regulated neuronal excitability through its activation by brain temperature in vivo. I recorded electroencephalogram (EEG) from wild type and TRPV4KO mice under free moving condition, and the EEG data revealed that hippocampal activities in TRPV4KO mice was significantly reduced compared with those in WT mice. Consistent with the EEG data, TRPV4KO hippocampal neurons exhibited abnormal excitatory postsynaptic potentials (EPSP) at physiological temperature. Taken together, we, for the first time, revealed that TRPV4 is an important translator from brain temperature to neuronal excitability in mammals.