Interaction between brain temperature and neuronal excitability

脑温度与神经元兴奋性之间的相互作用

• 批准号: 21200012
• 负责人: SHIBASAKI Koji
• 金额: $ 19.8万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21200012/
• 关键词: 脳内温度; TRPチャネル; TRPV4; 神経興奮; てんかん; 海馬; 体温; 産学官; 特許; メカノセンサー

Physiological brain temperature is an important determinant for neuronal functions, and it is well established that changes in temperature have dynamic influences on hippocampal neuronal activities. I previously revealed that a thermo-sensor TRPV4 (activated above 34°C) is activated by physiological brain temperature in hippocampal neurons and thereby controls their excitability in vitro. Therefore, it is considered that the thermo-sensor TRPV4 can convert temperature information to electrical excitability in mammalian brains. I examined whether TRPV4 regulated neuronal excitability through its activation by brain temperature in vivo. I recorded electroencephalogram (EEG) from wild type and TRPV4KO mice under free moving condition, and the EEG data revealed that hippocampal activities in TRPV4KO mice was significantly reduced compared with those in WT mice. Consistent with the EEG data, TRPV4KO hippocampal neurons exhibited abnormal excitatory postsynaptic potentials (EPSP) at physiological temperature. Taken together, we, for the first time, revealed that TRPV4 is an important translator from brain temperature to neuronal excitability in mammals.

生理脑温度是神经元功能的重要决定因素，并且已经确定温度的变化对海马神经元活动具有动态影响。我以前发现，热传感器TRPV4（在34°C以上激活）被海马神经元中的生理大脑温度激活，从而控制它们的体外兴奋性。因此，可以认为温度传感器TRPV4可以将温度信息转换为哺乳动物大脑中的电兴奋性。我研究了TRPV4是否通过体内脑温激活来调节神经元的兴奋性。在自由活动状态下记录野生型和TRPV4KO小鼠的脑电图（EEG），结果显示TRPV4KO小鼠海马活动较野生型小鼠明显减弱。与EEG数据一致，TRPV 4 KO海马神经元在生理温度下表现出异常的兴奋性突触后电位（EPSP）。综上所述，我们首次揭示了TRPV4是哺乳动物脑温度到神经元兴奋性的重要翻译者。

项目成果

Involvement of TRPV2 activation in intestinal movement through NO production in mice.

TRPV2 通过产生 NO 参与小鼠肠道运动的激活。

• 发表时间: 2010
• 期刊: The Journal of Neuroscience
• 作者: Mihara H;Budaka A;Shibasaki K;Yamanaka A;Sugiyama T;Tominaga M.
• 通讯作者: Tominaga M.

Characterization of astrocyte precursor cells in the developing mouse cerebellum.

发育中的小鼠小脑中星形胶质细胞前体细胞的表征。

• 发表时间: 2010
• 作者: Ishizaki Y;Masashi K;Cai N;Okano-Uchida T;Shibasaki K.
• 通讯作者: Shibasaki K.

A specific subtype of astrocytes regulates neuronal excitability through gliotransmitter release.

星形胶质细胞的特定亚型通过胶质递质释放调节神经元兴奋性。

• 发表时间: 2010
• 作者: 福原大裕;他;Shibasaki K
• 通讯作者: Shibasaki K

The TRPV4 Cation Channel Mediates Stretch-evoked Ca2+ Influx and ATP Release in Primary Urothelial Cell Cultures

• DOI: 10.1074/jbc.m109.020206
• 发表时间: 2009-08-07
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Mochizuki, Tsutomu;Sokabe, Takaaki;Tominaga, Makoto
• 通讯作者: Tominaga, Makoto

Activation of Polycystic Kidney Disease-2-like 1 (PKD2L1)-PKD1L3 Complex by Acid in Mouse Taste Cells

• DOI: 10.1074/jbc.c110.132944
• 发表时间: 2010-06-04
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Kawaguchi, Hitoshi;Yamanaka, Akihiro;Tominaga, Makoto
• 通讯作者: Tominaga, Makoto