权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of instructional method of the tailor-made exercise and diet therapies based on gene polymorphism for improvement of metabolic syndrome

基于基因多态性改善代谢综合征的量身定制的运动和饮食疗法指导方法的开发

基本信息

批准号：
20700563
负责人：
IEMITSU Motoyuki
金额：
$ 2.75万
依托单位：
International Pacific University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2009
项目状态：
已结题

项目摘要

The present study aimed to identify gene polymorphisms that provided for the behavior modification of the exercise and diet for improvement of metabolic syndrome, and these findings may contribute to basic developing of instructional method of the tailor-made exercise and diet therapies based on gene polymorphism. Firstly, we divided into two groups according to the evaluation of the exercise behavior modification (the physical activity measured by accelerometer) before and after intervention by the exercise guidance for 30-65 years old; the exercise behavior modification group (N=19) and the none exercise behavior modification group (N=13). To investigate comparison analysis of 550,000 gene polymorphisms using gene chip (Human660W-Quad Bead Chip made by the illumina company) between two groups, we have extracted genomic DNA from plasma buffy coats and buccal cells. The result using case-control analysis (p<0.0001) detected 5 SNPs from among 550,000 gene polymorphisms. A gene related to immunological system was included in 5 SNPs. Secondly, we examined to comparison analysis of 550,000 gene polymorphisms by using gene chip among four groups, large or little exercise behavior modification groups before and after intervention by the exercise guidance and high or low physical activity groups through one year (total N=112). The result using case-control analysis (p<0.0001) detected 16 SNPs from among 550,000 gene polymorphisms. Genes related to axon inducement and ion channel in cerebrum were included in 16 SNPs. Thus, these detected SNPs may affect the modification of exercise behavior.

本研究的目的是确定基因多态性，提供了运动和饮食改善代谢综合征的行为改变，这些发现可能有助于基础上的运动和饮食疗法的指导方法的基础上开发的基因多态性。首先，根据30-65岁老年人运动指导干预前后运动行为改变（加速度计测量的身体活动量）的评价分为两组：运动行为改变组（N=19）和非运动行为改变组（N=13）。为探讨基因芯片（Illumina公司生产的Human 660 W-Quad Bead Chip）对两组55万个基因多态性的比较分析，我们从血浆血沉棕黄层和口腔粘膜细胞中提取基因组DNA。病例对照分析结果表明，在55万个基因多态性位点中检出5个SNP位点（p<0.0001）。5个SNPs中含有1个免疫系统相关基因。第二，采用基因芯片技术对运动指导干预前后大、小运动行为改变组和高、低体力活动组（共112例）的55万个基因多态性进行比较分析。病例对照分析结果（p<0.0001），从55万个基因多态性中检出16个SNPs。16个SNP中包括与轴突诱导和大脑离子通道相关的基因。因此，这些检测到的SNPs可能会影响运动行为的改变。