Establishment of assay for bacterial contamination in source ofmineral water by using Limulus test.

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We developed the assay system for the bacterial contamination in the source of mineral water by applying the Limulus test. We studied 41 of Japanese mineral waters. We found that the contamination of bacteria which is equivalent to about 8.9×10^3~2.2×10^4cfu/mlfrom three mineral waters. Furthermore, we detected the gene of coliform bacteria in one of these three mineral waters. From these results, it was demonstrated that our assay system is very useful for the detection of bacterial contamination in the source of mineral water.