Development of a Technique for Quantitative Analysis of Heavy-ion Induced DNA Double-strand Breaks

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Biological effects of ionizing radiation per dose are larger in high-LET heavy ions than in low-LET radiations. As a reason of this phenomenon, radiation biologists believe that heavy ions induce localized DNA damage which is difficult to repair in irradiated cells. However, the conventional experimental techniques such as pulsed-field gel electrophoresis could not quantify radiation-induced DNA fragmentation that cuts genomic DNA shorter than 10 kbp. In the present study, I have found that most of DNA fragments shorter than 10 kbp were lost during genomic DNA preparation.