Highly sensitive detection of fluorescence with an optical waveguide substrate and polarized excitation light

使用光波导基板和偏振激发光进行荧光高灵敏度检测

• 批准号: 20710098
• 负责人: AKIMOTO Takuo
• 金额: $ 1.33万
• 依托单位: Tokyo University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20710098/
• 关键词: バイオチップ; 蛍光; バイオ関連機器; マイクロアレイ; プロテインチップ; 偏光

Fluorescence from an optical waveguide substrate fabricated with a metal and a dielectric was known to be enhanced more than 100 factors. It has been found in recent year that the fluorescence enhancement depends on the polarization of excitation light. Motivated by this finding, we attempted to develop an instrument for highly sensitive detection of fluorescence employing the polarization dependence of the optical waveguide substrate. As a result, approximately 10-times increase of the sensitivity and S/N ratio could be achieved.

已知来自用金属和电介质制造的光波导衬底的荧光增强超过100倍。近年来研究发现，荧光增强与激发光的偏振有关。基于这一发现，我们试图开发一种利用光波导基片的偏振依赖性进行高灵敏度荧光检测的仪器。结果，可以实现灵敏度和S/N比的大约10倍的增加。

项目成果

AgとAl203で作製した多層膜基板を用いた蛍光標識DNAの高感度検出

使用由 Ag 和 Al2O3 制成的多层基质对荧光标记 DNA 进行高灵敏度检测

• 发表时间: 2008
• 作者: 安田充;秋元卓央;軽部征夫;Biosensors 2008
• 通讯作者: Biosensors 2008

Highly sensitive detection of fluorescence by a plane metal su rface and a polarized excitation light

通过平面金属表面和偏振激发光对荧光进行高灵敏度检测

• 发表时间: 2008
• 作者: Takuo Akimoto;Mitsuru Yasuda;Isao Karube
• 通讯作者: Isao Karube

多層膜基板上での偏光した励起光の反射が与える蛍光増強への効果

偏振激发光在多层基底上的反射对荧光增强的影响

• 发表时间: 2009
• 作者: 安田充;秋元車央;軽部征夫
• 通讯作者: 軽部征夫

Fluorescence enhancement and reflection of the excitation light observed with a multilayered substrate

• DOI: 10.1364/ao.49.000080
• 发表时间: 2010-01-01
• 期刊: APPLIED OPTICS
• 影响因子: 1.9
• 作者: Akimoto, Takuo;Yasuda, Mitsuru
• 通讯作者: Yasuda, Mitsuru

Effect of the polarization and incident angle of excitation light on the fluorescence enhancement observed with a multilayered substrate fabricated by Ag and Al2O3.

激发光的偏振和入射角对 Ag 和 Al2O3 多层基板观察到的荧光增强的影响。

• DOI: 10.1364/ao.47.003789
• 发表时间: 2008
• 期刊: Applied optics
• 影响因子: 1.9
• 作者: T. Akimoto;Mitsuru Yasuda;I. Karube
• 通讯作者: I. Karube