RNAi screening for genes involved in differentiation of embryonic stem cells into primordial germ cells.

在这项研究中，我们利用小鼠胚胎干细胞作为胚胎发生的体外模型，并开发了一个RNAi筛选系统，用于识别参与原始生殖细胞发育的基因。我们对864个基因进行了初步筛选，并确定了5个候选基因。这些基因在ES细胞中的敲低导致生殖细胞特异性基因的表达显著增加，并且这些细胞似乎在一定程度上获得了晚期原始生殖细胞的性质。

In this study, we utilized mouse embryonic stem cells as an in vitro model for embryogenesis and developed an RNAi screening system for identifying genes involved in development of primordial germ cells. We performed a pilot screen of 864 genes and identified five candidate genes. Knockdown of these genes in ES cells led to a significant increase in expression of germ cell specific genes and these cells seemed to acquire properties of late stage primordial germ cells to some extent.