RNAi screening for genes involved in differentiation of embryonic stem cells into primordial germ cells.
RNAi screening for genes involved in differentiation of embryonic stem cells into primordial germ cells.
RNAi 筛选参与胚胎干细胞分化为原始生殖细胞的基因。
批准号：
20710151
负责人：
MAEDA Ikuma
金额：
$2.75万
依托单位：
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2008
资助国家：
日本
项目状态：
已结题
起止时间：
2008 至 2009
中文摘要
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英文摘要
In this study, we utilized mouse embryonic stem cells as an in vitro model for embryogenesis and developed an RNAi screening system for identifying genes involved in development of primordial germ cells. We performed a pilot screen of 864 genes and identified five candidate genes. Knockdown of these genes in ES cells led to a significant increase in expression of germ cell specific genes and these cells seemed to acquire properties of late stage primordial germ cells to some extent.
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会议论文
In vitro assay system for primordial germ cell development
原始生殖细胞发育的体外测定系统
原始生殖细胞发育的体外测定系统
DOI：
--
发表时间：
2009
期刊：
Cell Research 19
影响因子：
--
作者：
[Ikuma Maeda, Yasuhisa Matsui.]
通讯作者：
Yasuhisa Matsui.
RNAi screening for genes involved in differentiation of embryonic stem cells into primordial germ cells.
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批准号：22710197
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项目类别：Grant-in-Aid for Young Scientists (B)
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资助金额：$2.58万
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财政年份：2010
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负责人：MAEDA Ikuma
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依托单位：
海外基金
