Development of hydrogen-bonding lanthanide complex that recognizes nucleic acid base and Its application for the determination of DNA sequence

识别核酸碱基的氢键稀土配合物的研制及其在DNA序列测定中的应用

• 批准号: 20750055
• 负责人: YOSHIMOTO Keitaro
• 金额: $ 2.83万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20750055/
• 关键词: 核酸; 分子認識; ランタノイド錯体; 遺伝子診断・解析; 蛍光識別; エネルギー移動発光; 希土類錯体; 構造解析; 水素結合; 遺伝子診断

We newly synthesized a nucleobase-binding ligand, AMND-DOTA, in which 2-amino-5, 7-dimethyl-1, 8-naphthyridine (AMND) was conjugated with 1, 4, 7, 10-tetraazacyclododecane-1, 4, 7-triacetic acid (DOTA) via an amide linker, and found that its terbium(III) complex (AMND-DOTA-Tb) showed green emission based on an energy transfer from the naphthyridine moiety to Tb^<3+>. The nucleobase selectivity of AMND-DOTA was not changed by the conjugation of DOTA moiety to AMND, while the nucleobase-binding ability of AMND-DOTA-Tb disappeared. Finally, a mixed solution of AMND-DOTA and AMND-DOTA-Tb enabled the luminescence-based colorimetric discrimination of single-nucleotide transversions with the naked eye at a single excitation wavelength.

我们新合成了一种核碱基结合配体AMND-DOTA，其中2-氨基-5,7-二甲基-1,8-萘啶(AMND)通过酰胺连接体与1,4,7,10-四氮杂环十二烷-1,4,7-三乙酸(DOTA)缀合，并发现其铽(III)络合物 (AMND-DOTA-Tb)显示基于从萘啶部分到Tb ^ 3+ 的能量转移的绿色发射。 DOTA部分与AMND的缀合并未改变AMND-DOTA的核碱基选择性，而AMND-DOTA-Tb的核碱基结合能力消失。最后，AMND-DOTA 和 AMND-DOTA-Tb 的混合溶液能够在单一激发波长下用肉眼对单核苷酸颠换进行基于发光的比色区分。

项目成果

Synthesis of Functionalized Naphthyridine Derivatives and Its Application for Fluorescence-Based Colorimetric Discrimination of Single Nucleotide Transversion International Symposium on atomic technology

功能化萘啶衍生物的合成及其在荧光比色辨别单核苷酸转换中的应用原子技术国际研讨会

• 发表时间: 2009
• 作者: 厚見宙志;吉本敬太郎;齋藤伸吾;大熊盛也;前田瑞夫;長崎幸夫
• 通讯作者: 長崎幸夫

Synthesis of Functionalized Naphthyridine Derivatives and Its Application for Fluorescence-Based Colorimetric Discriminatio n of Single Nucleotide Transversion International Symposium on atomic technology

功能化萘啶衍生物的合成及其在荧光比色辨别单核苷酸转换中的应用原子技术国际研讨会

• 发表时间: 2009
• 作者: 厚見宙志;吉本敬太郎;齋藤伸吾;大熊盛也;前田瑞夫;長崎幸夫
• 通讯作者: 長崎幸夫

Luminescence-based colorimetric discrimination of single-nucleotide transversions by the combined use of the derivatives of DOTA-conjugated naphthyridine and its terbium complex

• DOI: 10.1016/j.tetlet.2009.02.152
• 发表时间: 2009-05-13
• 期刊: TETRAHEDRON LETTERS
• 影响因子: 1.8
• 作者: Atsumi, Hiroshi;Yoshimoto, Keitaro;Nagasaki, Yukio
• 通讯作者: Nagasaki, Yukio

Influence of substituent modifications on the binding of 2-amino-1,8-naphthyridines to cytosine opposite an AP site in DNA duplexes: thermodynamic characterization.

• DOI: 10.1093/nar/gkn1079
• 发表时间: 2009-04
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Sato Y;Nishizawa S;Yoshimoto K;Seino T;Ichihashi T;Morita K;Teramae N
• 通讯作者: Teramae N

Luminescence-based Cotorimetric Discrimination of Single-nucleotide Transversions by the Combined Use of the Derivatives of DOTA-conjugated Naphthyridine and Its Terbium Complex

结合使用 DOTA 共轭萘啶及其铽配合物衍生物对单核苷酸颠换进行基于发光的余度判别

• 发表时间: 2009
• 期刊: Tetrahedron Letters 50
• 作者: 厚見宙志;吉本敬太郎;齋藤伸吾;大熊盛也;前田瑞夫;長崎幸夫
• 通讯作者: 長崎幸夫