Direct protein analysis from electrophoresis gel using a mid-infrared laser

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In this study, I performed protein analysis from acrlamide gel after electrophoresis using a Mid-infrared tunable laser. I reported that the IR-MALDI process is influenced by the hydrogen bond around the carboxyl group or residual water. The aclylamide solution has a strong absorption bands at around 6.0μm correstponding to the >C=O stretching vibration mode. I obtained the mass spectrum of insulin ionized from acrylamide gel when the sample was irradiated by the infrared laser in a wavelength range of 5.8-5.9μm.