Identification and characterization of G-quadruplex DNA binding protein

我们报道了EWS中Arg-Gly-Gly结构域（RGG）与g-四重体DNA中富含g的单链DNA折叠结合，而不能与富含g的双链和单链DNA结合。在含有g -四重体的模板上，RGG通过形成稳定的g -四重体-RGG复合物，以RGG浓度的方式抑制DNA聚合酶。此外，RGG与g -四重体DNA的特异性结合是由RGG中精氨酸的氢键决定的。

We report that Arg-Gly-Gly domain (RGG) in EWS binds G-rich single strand DNA fold in G-quadruplex DNA while it cannot bind G-rich double and single stranded DNA. Inhibition of DNA polymerase on a template containing G-quadruplex in the presence of RGG occurred in an RGG concentration-manner by forming a stabilized G-quadruplex-RGG complex. Moreover,」 specific binding of RGG to G-quadruplex DNA is dictated by hydrogen bonding of arginine in RGG.