Detection of 5-methylcytosine in DNA by functionalized oligodeoxynucleotides

点击翻译按钮获取中文摘要

Although the NQ-photosensitized oxidation accompanied by selective strand cleavage at ^mC in DNA is an attractive method for identification of the methylation site in DNA, the relatively lower sensitivity of the detection method due to the less efficiency in the photooxidation of ^mC still remains as a major subject to be improved for establishing a status of general availability. In this study, we characterized reaction mechanism of the NQ-photosensitized one-electron oxidation of 5-methyl-2-deoxycytidine (d^mC) in order to obtain insight into the design of photochemical system involving the ^mC-selective strand cleavage with high sensitivity. We confirmed that deprotonation of d^mC radical cation and formation of methyl radical species are key reaction for the present photoreaction.