Experimental design of ELISA for quantitative MCT4 analysis and an investigation of signal transduction cascades

用于定量 MCT4 分析的 ELISA 实验设计和信号转导级联研究

• 批准号: 21500690
• 负责人: IIZUKA Kenji
• 金额: $ 2.91万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21500690/
• 关键词: 生活習慣病; 骨格筋細胞; MCT4; バイオマーカー; 抗体

Two types of antibodies, anti-monocarboxylate transporter(MCT) 4 polyclonal antibody recognizes C-terminal end of MCT4 and the anti-MCT4 polyclonal antibody created by considering synthetic peptide of the intermediate part of MCT4 molecule as an antigen, were prepared. Using these antibodies, we constructed both a single and a sandwiche enzyme-linked immunosorbent assay system for quantification of MCT4 concentration in serum and/or culture medium.

制备了识别MCT4 c末端的抗单羧酸转运蛋白（MCT） 4多克隆抗体和以MCT4分子中间部分合成肽为抗原制备的抗MCT4多克隆抗体。利用这些抗体，我们构建了单个和三明治酶联免疫吸附测定系统，用于血清和/或培养基中MCT4浓度的定量。

项目成果

エネルギー代謝指標としてのMCT4の有用性についての基礎的検討

MCT4作为能量代谢指标的实用性基础研究

• 发表时间: 2010
• 作者: 飯塚健治;森田憲輝;町田拓自;平藤雅彦
• 通讯作者: 平藤雅彦