Study for the correlation between lifestyle-related diseases and the inhibition of estrogen sulfo-conjugation by coffee.

我们发现，烘焙咖啡中的成分在人类胃肠道细胞模型Caco-2中强烈抑制雌激素的硫酸盐结合。我们假设，咖啡对雌激素硫化的抑制作用与日常饮用咖啡对生活方式相关疾病的预防作用有关。为了证明这一假设，我们试图提纯和鉴定咖啡中的抑制成分。从1公斤无咖啡因速溶咖啡中，成功地进行了成分的纯化和鉴定。该组分具有一种新型的含氮芳香结构(MW.205，C_&lt；11&gt；H_&lt；11&gt；NO_3)。我们还发现咖啡中的成分影响与雌激素代谢有关的蛋白质(SULT1E1、硫脂酶和BCRP)的基因表达，并分析了这种现象背后的机制。

We found that components in roasting coffee strongly inhibit the sulfate conjugation of estrogen in the cell model of human gastrointestinal tract, Caco-2. We hypothesized that this inhibitory effect of coffee on the estrogen sulfation is correlated to the preventive effect of daily coffee consumption on lifestyle-related diseases. To demonstrate this hypothesis, we tried to purify and identify the inhibitory components of coffee. From 1kg decaffeinated instant coffee, we succeeded in purification and identification of the component. The component has a novel nitrogen-containing aromatic structure(MW. 205, C_<11> H_<11> NO_3). We also found that components in coffee affect gene expression of proteins involved in estrogen metabolism(SULT1E1, sulfatase, and BCRP), and analyzed the mechanism underlying the phenomenon.