(-)-epigallocatechin-3-gallate protects against neuronal cell death and improves cerebralfunction after traumatic brain injury in rats

(-)-表没食子儿茶素-3-没食子酸酯可防止大鼠脑外伤后神经元细胞死亡并改善脑功能

• 批准号: 21500803
• 负责人: SATOU Takao
• 金额: $ 2.91万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21500803/
• 关键词: 脳外傷; 緑茶飲料; 神経保護作用; 酸化ストレス; EGCG; 緑茶; アポトーシス; 神経再生; 神経幹細胞; カテキン; 記憶改善

A major component of green tea, a widely consumed beverage, is(-)-epigallocatechin gallate(EGCG), which has strong antioxidant properties. In this study, we investigated the effects of EGCG on cerebral function and morphology following traumatic brain injury(TBI). Six-week-old male Wistar rats that had access to normal drinking water, or water containing 0.1%(w/v) EGCG ad libitum, received TBI with a pneumatic controlled injury device at 10 weeks of age. Immunohistochemistry and lipid peroxidation studies revealed that at 1, 3 and 7 days post-TBI, the number of 8-OHdG-, 4-HNE-and ssDNA-positive cells, and the levels of MDA around the damaged area after TBI, significantly decreased in the EGCG treatment group compared with the water group. In addition, there was a significant increase in the number of surviving neuronal cells and an improvement in cerebral dysfunction after TBI in the EGCG treatment group compared with the water group. In summary, consumption of green tea may be an effective therapy for TBI patients.

绿茶是一种被广泛消费的饮料，其主要成分是表没食子儿茶素没食子酸酯(EGCG)，它具有很强的抗氧化性能。在本研究中，我们观察了EGCG对创伤性脑损伤后脑功能和形态的影响。6周龄雄性Wistar大鼠饮用正常饮用水或含0.1%(w/v)EGCG的水，在10周龄时使用气动控制损伤装置进行颅脑损伤。免疫组织化学和脂质过氧化研究显示，EGCG治疗组脑损伤后1、3、7d，损伤区周围8-OHdG、4-HNE和ssDNA阳性细胞数及丙二醛含量均明显低于水组。此外，与水组相比，EGCG治疗组脑损伤后存活的神经细胞数量显著增加，脑功能障碍明显改善。综上所述，饮用绿茶可能是治疗脑外伤患者的一种有效方法。

项目成果

脳外傷損傷局所へのSDF1αとCXCR4アンタゴニスト投与による局所反応の変化に関する免疫組織化学的検討

SDF1α和CXCR4拮抗剂对脑外伤损伤区域局部反应变化的免疫组织化学研究

• 发表时间: 2009
• 作者: Itoh T;et al.
• 通讯作者: et al.

Increased apoptotic neuronal cell death And cerebral dysfunction after traumatic brain injury in aged rats

老年大鼠脑外伤后神经细胞凋亡增加和脑功能障碍

• 发表时间: 2013
• 期刊: Brain Struct Funct
• 影响因子: 3.1
• 作者: Itoh T;Imano M;Nishida S;Tsubaki M;Mizuguchi N;Hashimoto S;Ito A;Satou T
• 通讯作者: Satou T

脳卒中易発症性高血圧自然発症ラット(SHR3P)大脳における脳卒中発症後の神経再生に関する検討

脑卒中易发性高血压大鼠脑卒中后神经再生研究（SHR3P）

• 发表时间: 2009
• 作者: Satou;et al.
• 通讯作者: et al.

脳卒中発症後の神経幹細胞の出現へのSDF-1a/CXCR4系の関与に関する検討

SDF-1a/CXCR4系统参与脑卒中后神经干细胞出现的研究

• 发表时间: 2011
• 作者: 伊藤龍生;竹森久美子;橋本重夫;伊藤浩行;伊藤彰彦;佐藤隆夫
• 通讯作者: 佐藤隆夫

Exercise increases neural stem cell proliferation surrounding the area of damage following rat traumatic brain injury

• DOI: 10.1007/s00702-010-0495-3
• 发表时间: 2011-02-01
• 期刊: JOURNAL OF NEURAL TRANSMISSION
• 影响因子: 3.3
• 作者: Itoh, Tatsuki;Imano, Motohiro;Satou, Takao
• 通讯作者: Satou, Takao