Development of methods for analyzing fungal metabolites by using a portable ion mobility spectrometer under atmospheric pressure

生长早期真菌气味分析对文物保护具有重要意义。使用独特的真菌气味开发了检测真菌种类和生长阶段的分析方法[Takeuchi等人，Surf]。接口肛门。, 44(6), 694-698(2012)］. 采用固体空间微萃取法提取微生物挥发性有机物，并进行GC/MS分析。当真菌生长时，作为MVOCs排放的醇、酮和醛的数量增加了。倍半萜的检测对真菌种类的确定有一定的参考价值。研制了离子迁移谱仪（IMS）固相微萃取入口系统样机。并编制了通过气味的IMS光谱分析鉴定真菌种类的软件程序。

Fungal odor analysis in early growth stages is important for the conservation of cultural properties. The analytical methods for detecting fungal species and the stage of growth were developed using a distinctive fungal odor[Takeuchi et al, Surf. Interface Anal., 44(6), 694-698(2012)］. Microbial volatile organic compounds were extracted by solid space microextaction, followed by GC/MS analysis. While the fungi were growing, the amount of alcohols, ketones and aldehydes as MVOCs emitted increased. The detection of sesquiterpenes was useful for determining fungal species. The prototype of solid-phase microextraction inlet system available in an ion mobility spectrometer(IMS) was manufactured. Software programs were also made for identifying fungal species by IMS spectra analysis of the odor.