New principle of aromatic metabolism

芳香代谢新原理

• 批准号: 5378359
• 负责人: Professor Dr. Georg Fuchs
• 金额: --
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5378359?language=en
• 关键词: New; principle; aromatic; metabolism

Aromatic compounds constitute the second most important class of natural organic compounds, next to carbohydrates. The aerobic pathways for aromatic degradation are considered established. Molecular oxygen is used for cleavage of the stable carbon ring. All intermediates of the pathways are free. This project aims at elucidating a new principle of aerobic aromatic metabolism. Thereby, the ring is not cleaved by oxygen and the intermediates are bound as coenzyme A thiosters. The studied examples concern the metabolism of 2-aminobenzoate (anthranilate), benzoate and phenylacetate in various bacterial genera. Three peculiarities are of special interest, (1) the unexpected role of coenzyme A in activating the aromatic ring, (2) the novel oxygenases that hydroxylate and dearomatize the ring, (3) the enzymes which finally hydrolytically open the dearomatized ring.

芳香族化合物构成了天然有机化合物的第二大类，仅次于碳水化合物。认为芳烃降解的好氧途径已经确立。分子氧用于裂解稳定的碳环。所有途径的中间体都是自由的。本项目旨在阐明有氧芳香代谢的新原理。因此，环不被氧裂解，中间体作为辅酶A硫酯结合。所研究的实例涉及2-氨基苯甲酸盐（邻氨基苯甲酸盐）、苯甲酸盐和苯乙酸盐在各种细菌属中的代谢。有三个特性特别令人感兴趣，（1）辅酶A在激活芳环中的意想不到的作用，（2）使环羟基化和脱芳构化的新型加氧酶，（3）最终水解打开脱芳构化环的酶。