Elucidation of new autotrophic CO2 fixation pathways in bacteria

阐明细菌中新的自养二氧化碳固定途径

• 批准号: 5377683
• 负责人: Professor Dr. Georg Fuchs
• 金额: --
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5377683?language=en
• 关键词: Elucidation; new; autotrophic; CO2; fixation

Autotrophy is the use of carbon dioxide (CO2) as sole source of cell carbon, and autotrophic CO2-fixation into organic compounds is the quantitatively most important life process. This capability is found not only in plants but also in most major groups of bacteria. Three autotrophic pathways have been established, the Calvin cycle, the reductive citric acid cycle, and the reductive acetyl-CoA pathway. This project aims at studying new CO2-fixation pathways in bacteria. The first goal is the elucidation of the proposed 3-hydroxypropionate cycle which functions as autotrophic pathway in the phototrophic eubacterium Chloroflexus aurantiacus. The genome of this model organism has been sequenced. The second aim is the study of similar but modified cycle in thermophilic Crenarchaeota. This group of archaebacteria may harbor other autotrophic pathways which will be studied. This project is expected to provide new insights into the physiology and metabolism of various bacteria. Furthermore, the discovery of new enzymes and pathways will have an impact on biochemistry and evolutionary biology. Finally, some applications can be envisaged.

自养是利用二氧化碳(CO2)作为细胞碳的唯一来源，而自养CO2固定成有机化合物是数量上最重要的生命过程。这种能力不仅在植物中发现，而且在大多数主要细菌群体中也存在。已经建立了三条自养途径，即Calvin循环、还原柠檬酸循环和还原乙酰-辅酶A途径。该项目旨在研究细菌中固定二氧化碳的新途径。第一个目标是阐明所提出的3-羟基丙酸循环，该循环在光营养真细菌橙色绿弯杆菌中起着自养途径的作用。这种模式生物的基因组已经被测序。第二个目的是研究嗜热性克伦考古群中相似但经过修改的循环。这组古细菌可能含有其他自养途径，这些途径将被研究。该项目有望为各种细菌的生理学和新陈代谢提供新的见解。此外，新的酶和途径的发现将对生物化学和进化生物学产生影响。最后，可以设想一些应用。